IFA1 Porcine
HEK293 cells.
Interferon alpha-1, Interferon-alpha, IFN-alpha-1, IFNA1, IFN-ALPHA-1, IFN1
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
IFA1 porcine Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 24-189) containing 176 amino acids and having a molecular mass of 20.2kDa.
IFA1 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Interferon alpha-1, Interferon-alpha, IFN-alpha-1, IFNA1, IFN-ALPHA-1, IFN1
HEK293 cells.
DGSMCDLPQT HSLAHTRALR LLAQMRRISP FSCLDHRRDF GSPHEAFGGN QVQKAQAMAL VHEMLQQTFQ LFSTEGSAAA WNESLLHQFC TGLDQQLRDL EACVMQEAGL EGTPLLEEDS ILAVRKYFHR LTLYLQEKSY SPCAWEIVRA EVMRSFSSSR NLQDRLRKKE HHHHHH
Product Science Overview
Interferon-alpha (IFN-α) is a type I interferon, a group of cytokines critical for the host’s immune response against viral infections. Porcine interferon-alpha (PoIFN-α) is derived from pigs and has been extensively studied for its antiviral properties and potential applications in veterinary medicine and biomedical research.
The porcine type I interferon family, including IFN-α, has undergone significant molecular and functional diversification due to the evolutionary pressures associated with the speciation and domestication of pigs . The current swine genome assembly reveals 57 functional genes and 16 pseudogenes of type I IFNs, with multiple subfamilies of IFN-α, IFN-ω, and porcine-specific IFN-δ genes . This diversification is driven by gene duplication, conversion, and natural selection, resulting in a wide range of antiviral activities and expression profiles among different IFN subtypes .
Porcine IFN-α is primarily produced by plasmacytoid dendritic cells (pDCs) in response to pathogenic infections . The recombinant form of PoIFN-α, known as Interferon-alpha 1 Porcine Recombinant, is produced using genetic engineering techniques, typically in bacterial or mammalian expression systems . This recombinant protein retains the antiviral properties of the native cytokine and has been shown to inhibit the replication of various viruses, including vesicular stomatitis virus (VSV) and pseudorabies virus (PRV) .
The structure-activity relationship of porcine IFN-α has been studied extensively to understand its antiviral mechanisms . The protein’s 3-D structure reveals key regions responsible for its interaction with the interferon receptors (IFNAR1 and IFNAR2) and subsequent activation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway . This pathway leads to the expression of hundreds of interferon-stimulated genes (ISGs) that establish an antiviral state within the host cells .
Recombinant porcine IFN-α has shown promise in veterinary clinical applications, particularly in controlling viral infections in swine populations . Its ability to modulate the immune response and enhance antiviral defenses makes it a valuable tool for both therapeutic and prophylactic purposes. Additionally, the study of porcine IFN-α provides insights into the broader family of type I interferons and their roles in immune regulation and antiviral defense .
