IFIH1 Human
Greater than 93.0% as determined by SDS-PAGE.
Description
IFIH1 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 152,000 Dalton. IFIH1 is expressed with a -10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interferon Induced With Helicase C Domain 1 (IFIH1), also known as Melanoma Differentiation-Associated Protein 5 (MDA5), is a protein encoded by the IFIH1 gene in humans. This protein plays a crucial role in the innate immune response by acting as a cytoplasmic sensor of viral RNA. Upon detecting viral RNA, IFIH1 triggers a cascade of antiviral responses, including the production of type I interferons and pro-inflammatory cytokines .
IFIH1 is characterized by a modified DExD/H-box helicase core and a C-terminal domain, which are essential for its function as an RNA sensor. The protein binds to double-stranded RNA (dsRNA) oligonucleotides, recognizing specific RNA lengths and secondary structures. This binding leads to the activation of downstream signaling pathways that result in the production of interferons .
IFIH1 is a key player in the body’s defense against viral infections. It detects viral RNA from various viruses, including Picornaviridae family members (such as encephalomyocarditis virus and rhinovirus), coronaviruses (including SARS-CoV-2), dengue virus, West Nile virus, and reovirus . Upon binding to viral RNA, IFIH1 associates with the mitochondrial antiviral signaling protein (MAVS), which activates kinases like TBK1 and IKBKE. These kinases phosphorylate interferon regulatory factors (IRF3 and IRF7), leading to the transcription of antiviral genes .
