IER3 Human

Immediate Early Response 3 Human Recombinant
Cat. No.
BT15663
Source
Escherichia Coli.
Synonyms
immediate early response 3, DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1, Protein DIF-2, Immediate early protein GLY96, PACAP-responsive gene 1 protein, Differentiation-dependent gene 2 protein, IER3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

IER3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 105 amino acids (1-82 a.a.) and having a molecular mass of 11.4kDa.
IER3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The Immediate Early Response 3 (IER3) protein plays a crucial role in safeguarding cells against apoptosis triggered by Fas or tumor necrosis factor type alpha. It participates in the ERK signaling pathway by preventing the dephosphorylation of ERK by the phosphatase PP2A-PPP2R5C holoenzyme. Additionally, IER3 acts as an effector downstream of ERK, mediating cell survival. As a component of the NUPR1/RELB/IER3 survival pathway, IER3 confers significant resistance to pancreatic ductal adenocarcinoma cells against stress conditions such as starvation.
Description
Recombinant human IER3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 105 amino acids (specifically, amino acids 1-82). It has a molecular mass of 11.4 kDa. A 23 amino acid His-tag is fused to the N-terminus of IER3. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear solution, sterilized by filtration.
Formulation
The IER3 protein solution (0.25 mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 50% glycerol, and 2mM DTT.
Stability
For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 85.0%.
Synonyms
immediate early response 3, DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1, Protein DIF-2, Immediate early protein GLY96, PACAP-responsive gene 1 protein, Differentiation-dependent gene 2 protein, IER3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMCHSRSC HPTMTILQAP TPAPSTIPGP RRGSGPEIFT FDPLPEPAAA PAGRPSASRG HRKRSRRVLY PRVVRRQLPV EEPNP.

Product Science Overview

Gene and Protein Structure

IER3 is located on chromosome 6 in humans and encodes a protein that is approximately 16.9 kDa in size . The protein is primarily expressed in the cytoplasm of cells and has been identified in various tissues, including the liver, kidney, and heart .

Function and Mechanism

The IER3 protein exerts diverse effects on cellular processes. It can function as both an oncogene and a tumor suppressor, depending on the type of cancer . In some cancers, such as bladder cancer, increased expression of IER3 is associated with aggressive progression and poor prognosis . The protein is involved in regulating apoptosis by interacting with key signaling pathways, including the NF-κB and MAPK pathways .

Clinical Significance

IER3 has been studied extensively for its role in cancer biology. In bladder cancer, high expression of IER3 is significantly associated with advanced disease stages and shorter overall survival . This makes IER3 a potential prognostic marker for bladder cancer patients . Additionally, IER3 is being explored as a therapeutic target, with research focusing on modulating its expression to control cancer progression .

Research and Applications

IER3 is widely used in research to study its role in various diseases and cellular processes. Recombinant IER3 protein, expressed in systems like HEK293 cells, is available for research purposes . This recombinant protein is used in various assays, including ELISA, to quantify IER3 levels in different samples .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.