IDNK E.Coli
Description
Product Specs
Product Science Overview
The recombinant form of thermosensitive gluconokinase is typically expressed in E. coli K-12 strains. The protein consists of 187 amino acids and is often tagged with a His-tag at the N-terminus to facilitate purification . The molecular mass of the recombinant protein is approximately 23.4 kDa . The protein is produced as a single, non-glycosylated polypeptide chain .
The recombinant thermosensitive gluconokinase is purified using proprietary chromatographic techniques to achieve a purity greater than 95% . The protein is supplied in a sterile filtered colorless solution containing 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT . For long-term storage, it is recommended to store the protein at -20°C and to avoid multiple freeze-thaw cycles .
Thermosensitive gluconokinase is biologically active and suitable for various applications, including SDS-PAGE, functional studies (FuncS), and mass spectrometry (MS) . The enzyme’s activity is crucial for the proper functioning of the Entner-Doudoroff pathway, which is essential for the metabolism of gluconate in E. coli.
Recombinant thermosensitive gluconokinase is used in research to study the metabolic pathways of E. coli and other bacteria. It is also employed in various biochemical assays to understand the enzyme’s kinetics and regulation. The high purity and activity of the recombinant protein make it suitable for detailed structural and functional analyses.
