IDH3G is a subunit of the isocitrate dehydrogenase enzyme complex, which catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate (alpha-ketoglutarate) while reducing NAD+ to NADH . This reaction is a critical step in the citric acid cycle, contributing to cellular respiration and energy production.
The human recombinant form of IDH3G is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 375 amino acids. It has a molecular mass of approximately 41.1 kDa and is fused to a 21 amino acid His-tag at the N-terminus . This recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and functionality .
IDH3G is essential for the proper functioning of the citric acid cycle. The enzyme’s activity is tightly regulated to maintain metabolic balance within the cell. Mutations or dysregulation of IDH3G can lead to metabolic disorders and have been associated with various diseases, including D-2-Hydroxyglutaric Aciduria 2 and Pseudopseudohypoparathyroidism .
The recombinant form of IDH3G is widely used in research to study its structure, function, and role in metabolism. It is also utilized in drug discovery and development, particularly in the context of metabolic diseases and cancer research. The availability of high-purity recombinant IDH3G allows scientists to conduct detailed biochemical and biophysical analyses, facilitating a deeper understanding of its mechanisms and interactions.
The recombinant IDH3G protein is typically stored at -20°C for long-term preservation. It is recommended to add a carrier protein, such as human serum albumin (HSA) or bovine serum albumin (BSA), to prevent degradation during storage. The protein should be handled with care to avoid multiple freeze-thaw cycles, which can affect its stability and activity .