ICT1 Human

Immature Colon Carcinoma Transcript 1 Human Recombinant
Cat. No.
BT15597
Source
Escherichia Coli.
Synonyms
DS-1, DS1, Peptidyl-tRNA hydrolase ICT1 mitochondrial, Digestion substraction 1, Immature colon carcinoma transcript 1 protein, ICT1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ICT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids (30-206 a.a) and having a molecular mass of 22.8kDa. ICT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Immature Colon Carcinoma Transcript 1 (ICT1) is a codon-independent translation release factor that has lost its specificity for stop codons. It plays a crucial role in terminating mitochondrial translation during instances of stalled elongation. In the mature colon epithelium, three distinct cell types originate from multipotent stem cells. ICT1 participates in hydrolyzing prematurely terminated peptidyl-tRNAs, thereby enabling the recycling of stalled mitochondrial ribosomes. Deviations from the normal cellular maturation process, leading to neoplastic transformation, are believed to originate in stem cells or their early progeny.
Description
Recombinant ICT1 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 200 amino acids (residues 30-206). It has a molecular weight of 22.8 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The ICT1 protein solution has a concentration of 0.5 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the protein can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure long-term stability during frozen storage, consider adding a carrier protein such as HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
DS-1, DS1, Peptidyl-tRNA hydrolase ICT1 mitochondrial, Digestion substraction 1, Immature colon carcinoma transcript 1 protein, ICT1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLHKQKDG TEFKSIYSLD KLYPESQGSD TAWRVPNGAK QADSDIPLDR LTISYCRSSG PGGQNVNKVN SKAEVRFHLA TAEWIAEPVR QKIAITHKNK INRLGELILT SESSRYQFRN LADCLQKIRD MITEASQTPK EPTKEDVKLH RIRIENMNRE RLRQKRIHSA VKTSRRVDMD.

Product Science Overview

Role in Cancer

ICT1 has been recognized for its overexpression in several cancer types, such as hepatoblastoma, glioblastoma multiforme, and non-small cell lung cancer . In colorectal cancer (CRC), ICT1 expression correlates with unfavorable prognosis and reduced survival rates . Studies have shown that ICT1 promotes CRC growth through intracellular signaling pathways, including AMPK, SAPK/JNK, and PARP .

Mechanism of Action

ICT1 functions as a component of the mitochondrial ribosome (mitoribosome) and exhibits peptidyl-tRNA hydrolase (PTH) activity via the tripeptide motif GGQ . This activity is crucial for maintaining mitochondrial protein synthesis and overall cellular function. Depletion of ICT1 disrupts mitoribosomal structure, leading to decreased mitochondrial membrane potential and mass .

Therapeutic Potential

Research has demonstrated that knockdown of ICT1 induces suppression of cell proliferation, S-phase arrest, and apoptosis in leukemia cells . This suggests that ICT1 could be a potential therapeutic target for treating various cancers. In CRC, ICT1 silencing has been shown to lower cell viability, inhibit cell migration, and induce apoptosis .

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Source
THE BIOTEK
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