HYKK Human
Description
HYKK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Hydroxylysine kinase (HYKK) is an enzyme that plays a crucial role in the metabolism of lysine, an essential amino acid. This enzyme is responsible for the phosphorylation of 5-hydroxy-L-lysine, a post-translationally modified form of lysine, using GTP as a phosphate donor . The human recombinant form of this enzyme is produced through recombinant DNA technology, allowing for its study and application in various research and therapeutic contexts.
The HYKK gene, also known as AGPHD1 (Aminoglycoside Phosphotransferase Domain-Containing Protein 1), is located on chromosome 15 in humans . The gene encodes a protein that is involved in the lysine degradation pathway, specifically in the saccharopine pathway . The enzyme is predicted to be located in the mitochondrial matrix, where it catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine .
Hydroxylysine kinase catalyzes the following chemical reaction: [ \text{GTP} + \text{5-hydroxy-L-lysine} \rightarrow \text{GDP} + \text{5-phosphonooxy-L-lysine} ] This reaction is essential for the metabolism of hydroxylysine, converting it into a phosphorylated form that can be further processed by other enzymes .
The phosphorylation of hydroxylysine by HYKK is a critical step in the lysine degradation pathway. This pathway is important for the catabolism of lysine, which is necessary for maintaining amino acid balance and producing energy in the form of ATP . Additionally, the products of lysine degradation are involved in various metabolic processes, including the synthesis of carnitine, a molecule essential for fatty acid metabolism .
The human recombinant form of hydroxylysine kinase is produced using recombinant DNA technology. This involves inserting the HYKK gene into a suitable expression vector, which is then introduced into a host organism, such as E. coli or yeast. The host organism expresses the HYKK protein, which can be purified and used for research or therapeutic purposes .
Recombinant hydroxylysine kinase is valuable for studying the enzyme’s structure, function, and role in lysine metabolism. It can also be used to investigate the molecular mechanisms underlying certain metabolic disorders, such as 5-hydroxylysinuria, which is characterized by the accumulation of hydroxylysine in the body . Furthermore, understanding the function of HYKK may provide insights into the development of new therapeutic strategies for treating metabolic diseases.
