HUS1 Human
Description
HUS1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The HUS1 checkpoint homolog is a crucial component of the DNA damage response system in human cells. It plays a significant role in maintaining genomic stability by participating in the cell cycle checkpoint pathways. This article delves into the background, structure, function, and significance of the HUS1 checkpoint homolog, particularly focusing on its recombinant form.
HUS1 is a protein-coding gene that encodes a component of the 9-1-1 (RAD9-RAD1-HUS1) complex . This trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and is involved in the DNA damage response . The HUS1 protein itself is composed of 280 amino acids and has a molecular weight of approximately 33.8 kDa .
The primary function of HUS1 is to act as a checkpoint protein that helps in the detection and repair of DNA damage. In response to genotoxic stress, HUS1 forms a heterotrimeric complex with RAD9 and RAD1 . This 9-1-1 complex is recruited to sites of DNA lesions by the RAD17-replication factor C (RFC) clamp loader complex . Once localized to the DNA damage sites, the 9-1-1 complex acts as a sliding clamp platform for several proteins involved in long-patch base excision repair (LP-BER) .
HUS1 is an evolutionarily conserved protein that plays a pivotal role in the DNA damage response. Upon DNA damage, the 9-1-1 complex is loaded onto the chromatin, which is an early checkpoint signaling event . This process is dependent on the activation of the checkpoint kinase ATM . The complex then facilitates DNA damage signaling, repair, or apoptosis, and cell cycle arrest .
Mutations or dysregulation of HUS1 can lead to genomic instability and are associated with various diseases. For instance, HUS1 has been linked to Noonan Syndrome 2 and Deafness, Autosomal Recessive 4, with Enlarged Vestibular Aqueduct . Understanding the function and regulation of HUS1 is crucial for developing therapeutic strategies for diseases resulting from genomic instability.
Recombinant HUS1 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the HUS1 gene into a suitable expression system, such as E. coli, to produce the protein in large quantities . Recombinant HUS1 is used in various research applications to study its function, interactions, and role in the DNA damage response.
