VEGFR2 Human, His

Vascular Endothelial Growth Factor receptor-2 Human Recombinant, His Tag
Cat. No.
BT21961
Source

Sf9, Baculovirus cells.

Synonyms

KDR D1-7, sKDR D1-7, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, CD309, type III receptor tyrosine kinase, FLK1, VEGFR-2. 

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

VEGFR2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 987 amino acids (20-764a.a) and having a molecular mass of 110.5kDa (Molecular size on SDS-PAGE will appear at approximately 100-150kDa). VEGFR2 is fused to a 239 amino acids hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vascular endothelial growth factor (VEGF) receptors are primarily found on endothelial cells and play a crucial role in angiogenesis (blood vessel formation). VEGFR-2, in particular, exhibits strong signaling activity upon VEGF binding, leading to endothelial cell proliferation. There are three main types of VEGF receptors (VEGFR-1, VEGFR-2, and VEGFR-3), each with distinct characteristics and functions.
Description
Recombinant human VEGFR2, produced using Sf9 insect cells, is a glycosylated protein with a molecular weight of approximately 110.5 kDa. To facilitate purification and detection, it includes a Histidine tag fused to the C-terminus of a 239 amino acid human IgG tag. The protein encompasses amino acids 20-764 of the VEGFR2 sequence. On SDS-PAGE, the apparent molecular weight may appear between 100-150 kDa due to glycosylation.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The VEGFR2 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is buffered with Phosphate Buffered Saline (pH 7.4) and contains 10% glycerol.
Stability
For short-term storage (up to four weeks), the VEGFR2 solution can be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) can enhance stability during long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the VEGFR2 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms

KDR D1-7, sKDR D1-7, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, CD309, type III receptor tyrosine kinase, FLK1, VEGFR-2. 

Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ADPASVGLPS VSLDLPRLSI QKDILTIKAN TTLQITCRGQ RDLDWLWPNN QSGSEQRVEV TECSDGLFCK TLTIPKVIGN DTGAYKCFYR ETDLASVIYV YVQDYRSPFI ASVSDQHGVV YITENKNKTV VIPCLGSISN LNVSLCARYP EKRFVPDGNR ISWDSKKGFT IPSYMISYAG MVFCEAKIND ESYQSIMYIV VVVGYRIYDV VLSPSHGIEL SVGEKLVLNC TARTELNVGI DFNWEYPSSK HQHKKLVNRD LKTQSGSEMK KFLSTLTIDG VTRSDQGLYT CAASSGLMTK KNSTFVRVHE KPFVAFGSGM ESLVEATVGE RVRIPAKYLG YPPPEIKWYK NGIPLESNHT IKAGHVLTIM EVSERDTGNY TVILTNPISK EKQSHVVSLV VYVPPQIGEK SLISPVDSYQ YGTTQTLTCT VYAIPPPHHI HWYWQLEEEC ANEPSQAVSV TNPYPCEEWR SVEDFQGGNK IEVNKNQFAL IEGKNKTVST LVIQAANVSA LYKCEAVNKV GRGERVISFH VTRGPEITLQ PDMQPTEQES VSLWCTADRS TFENLTWYKL GPQPLPIHVG ELPTPVCKNL DTLWKLNATM FSNSTNDILI MELKNASLQD QGDYVCLAQD RKTKKRHCVV RQLTVLERVA PTITGNLENQ TTSIGESIEV SCTASGNPPP QIMWFKDNET LVEDSGIVLK DGNRNLTIRR VRKEDEGLYT CQACSVLGCA KVEAFFIIEG AQEKTNLELE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.

Product Science Overview

Introduction

Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2), also known as Kinase Insert Domain Receptor (KDR) or Fetal Liver Kinase 1 (FLK1), is a type III receptor tyrosine kinase. It plays a crucial role in the regulation of angiogenesis, vascular development, and endothelial cell functions .

Structure and Expression

VEGFR-2 is a cell-surface receptor for VEGFA, VEGFC, and VEGFD. It is predominantly expressed in vascular endothelial cells and is an early marker for endothelial cell progenitors . The receptor consists of an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular tyrosine kinase domain .

Function

VEGFR-2 is the primary mediator of VEGF-induced endothelial proliferation, survival, migration, and tubular morphogenesis . It promotes the reorganization of the actin cytoskeleton and plays an essential role in embryonic hematopoiesis . The signaling and trafficking of VEGFR-2 are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, and T-cell protein tyrosine phosphatase .

Recombinant VEGFR-2 (Human, His Tag)

The recombinant form of VEGFR-2, tagged with a His (histidine) tag, is produced using insect cells as the expression system . This recombinant protein is purified and used in various research applications, including kinase assays and studies on VEGF signaling pathways . The His tag facilitates the purification process and allows for easy detection and quantification of the protein.

Applications

Recombinant VEGFR-2 (Human, His Tag) is widely used in research to study the mechanisms of angiogenesis and vascular development. It is also utilized in drug discovery and development, particularly in the context of anti-angiogenic therapies for cancer and other diseases .

Storage and Handling

The recombinant VEGFR-2 protein is typically stored at -80°C to maintain its stability and activity. It is important to avoid freeze/thaw cycles to prevent protein degradation .

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