HTRA2 Antibody

HTRA2, Mouse Anti Human
Cat. No.
BT19936
Source
Synonyms
Serine protease HTRA2 mitochondrial, EC 3.4.21.108, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine proteinase OMI, Serine protease 25, OMI, PARK13, PRSS25.
Appearance
Sterile filtered colorless solution.
Purity
Usage
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Description

Product Specs

Introduction
HtrA2, also known as Omi, is a mammalian serine protease that exhibits protease activity at high temperatures and chaperone activity at low temperatures. Initially synthesized as a precursor protein, full-length HtrA2 is targeted to the mitochondria, where it undergoes maturation through the removal of its N-terminal 133 residues. The mature HtrA2 protein comprises several key domains: a putative transmembrane domain, an inhibitor of apoptosis protein (IAP)-binding motif, and a single C-terminal PDZ domain responsible for mediating protein-protein interactions. Recent studies have highlighted the role of HtrA2 in both caspase-dependent and caspase-independent cell death pathways.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The solution is at a concentration of 1 mg/ml and contains phosphate-buffered saline (PBS) at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The HTRA2 antibody has undergone rigorous testing by ELISA, Western blot analysis, and ICC/IF to ensure its specificity and reactivity. As applications can vary, it is recommended to determine the optimal working dilution for each experiment. For Western blot analysis, a dilution range of 1:500 is suggested, while for ICC/IF, a dilution of 1:100 is recommended.
Synonyms
Serine protease HTRA2 mitochondrial, EC 3.4.21.108, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine proteinase OMI, Serine protease 25, OMI, PARK13, PRSS25.
Purification Method
HTRA2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone

PAT4G8AT.

Immunogen
Anti-human HTRA2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HTRA2 protein 134-458 amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Introduction

HTRA2, also known as Omi, is a serine protease located in the mitochondria. It plays a crucial role in the regulation of apoptosis, a process of programmed cell death essential for maintaining cellular homeostasis. The HTRA2 protein is encoded by the HTRA2 gene in humans and has homologs in other species, including mice and rats.

Structure and Function

HTRA2 is a mitochondrial protein that is released into the cytoplasm in response to apoptotic stimuli. It contains a serine protease domain and a PDZ domain, which are essential for its proteolytic activity and protein-protein interactions, respectively. The mature form of HTRA2 has a conserved IAP-binding motif (AVPS) at its N-terminus, which allows it to interact with and inhibit Inhibitor of Apoptosis Proteins (IAPs) .

Role in Apoptosis

HTRA2 is involved in the mitochondrial apoptotic pathway. Upon induction of apoptosis, HTRA2 is released from the mitochondria into the cytoplasm, where it binds to IAPs and neutralizes their anti-apoptotic effects. This interaction promotes the activation of caspases, the executioners of apoptosis, leading to cell death .

HTRA2 in Disease

Mutations or dysregulation of HTRA2 have been linked to various diseases, including neurodegenerative disorders such as Parkinson’s disease. Mice lacking HTRA2 exhibit a parkinsonian phenotype, suggesting a role for HTRA2 in the progression of Parkinson’s disease in humans and mice .

Mouse Anti-Human HTRA2 Antibodies

Mouse anti-human HTRA2 antibodies are used in various scientific applications to detect and study HTRA2 in human samples. These antibodies are typically monoclonal or polyclonal and are developed in mice. They can be used in techniques such as Western Blot, Immunohistochemistry, ELISA, Immunocytochemistry, and Immunoprecipitation .

Applications
  1. Western Blot: Used to detect HTRA2 protein levels in cell lysates.
  2. Immunohistochemistry: Allows visualization of HTRA2 expression in tissue sections.
  3. ELISA: Quantifies HTRA2 protein levels in various samples.
  4. Immunocytochemistry: Visualizes HTRA2 in cultured cells.
  5. Immunoprecipitation: Isolates HTRA2 protein complexes from cell lysates .

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