HSPB3 Human
Heat Shock 27kDa Protein 3 Human Recombinant
Cat. No.
BT18516
Source
E.coli.
Synonyms
Heat shock 27kDa protein 3, HSPL27, Protein 3, HSP 17, DHMN2C, HMN2C, heat shock protein beta-3, HspB3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HSPB3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (1-150) and having a molecular mass of 19.1 kDa.
The HSPB3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
The HSPB3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
HSPB3, primarily found in heart and skeletal muscle, is a small heat shock protein specific to muscle tissue. Belonging to a family of highly expressed proteins, mammalian small heat shock proteins (sHSPs) play diverse roles. These roles include the assembly and sequestration of multi-protein complexes, facilitating the transport of newly synthesized polypeptide chains across cell membranes, and modulating protein folding processes. HSPB3 functions as an ATP-dependent chaperone protein, actively participating in the refolding of misfolded proteins and regulating actin polymerization.
Description
Recombinantly produced in E. coli, HSPB3 Human Recombinant is a single, non-glycosylated polypeptide chain consisting of 170 amino acids (residues 1-150) and possessing a molecular weight of 19.1 kDa. For purification purposes, a 20 amino acid His-Tag is fused to the N-terminus of HSPB3, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The HSPB3 solution is provided at a concentration of 1mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 1M Urea, and 5% glycerol.
Stability
For short-term storage (2-4 weeks), the entire vial can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
SDS-PAGE analysis indicates a purity exceeding 85%.
Synonyms
Heat shock 27kDa protein 3, HSPL27, Protein 3, HSP 17, DHMN2C, HMN2C, heat shock protein beta-3, HspB3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAKIILRHLI EIPVRYQEEF EARGLEDCRL DHALYALPGP TIVDLRKTRA AQSPPVDSAA ETPPREGKSH FQILLDVVQF LPEDIIIQTF EGWLLIKAQH GTRMDEHGFI SRSFTRQYKL PDGVEIKDLS AVLCHDGILV VEVKDPVGTK
Product Science Overview
Classification and Structure
Biological Properties and Functions
Mode of Action
Regulatory Mechanisms
The expression of HSPB3 is regulated by various stress conditions. Heat shock proteins, including HSPB3, are typically upregulated in response to increased temperatures, oxidative stress, and other environmental stressors. This upregulation is mediated by heat shock factors (HSFs), which are transcription factors that bind to heat shock elements (HSEs) in the promoter regions of heat shock protein genes .
Clinical Significance
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