HSP70 Antibody
Description
Product Specs
Product Science Overview
The Heat Shock 70 kDa Protein (Hsp70) is a member of the heat shock protein family, which plays a crucial role in protein homeostasis. These proteins are highly conserved across different species, indicating their fundamental importance in cellular processes . Hsp70 proteins are involved in various cellular functions, including protein folding, protection against stress, and assisting in the degradation of misfolded proteins .
Structure and Function
Hsp70 proteins consist of three major functional domains:
- N-terminal ATPase Domain: This domain binds and hydrolyzes ATP (Adenosine triphosphate) to ADP (Adenosine diphosphate), which is essential for the protein’s chaperone activity .
- Substrate Binding Domain (SBD): Located at the C-terminal, this domain interacts with extended polypeptides as substrates .
- EEVD Motif: This motif at the C-terminal participates in binding to co-chaperones and other heat shock proteins .
Hsp70 proteins are upregulated in response to various stress conditions, such as heat shock, heavy metals, and toxic chemicals . This upregulation helps protect cells from adverse effects by stabilizing unfolded or misfolded proteins and preventing their aggregation .
Discovery
The heat shock response was first discovered by Ferruccio Ritossa in the 1960s when a lab worker accidentally increased the incubation temperature of Drosophila (fruit flies). This led to the observation of a “puffing pattern” in the chromosomes, indicating elevated gene transcription of an unknown protein, later identified as heat shock proteins .
The Mouse Anti-Human Hsp70 antibody is a monoclonal antibody that specifically targets the Hsp70 protein in humans. This antibody is widely used in various research applications, including immunohistochemistry, immunoprecipitation, flow cytometry, western blotting, and immunocytochemistry .
Characteristics
- Host Species: Mouse
- Isotype: IgG1
- Immunogen: Recombinant fragment protein within Human HSPA1A amino acids 100-300 .
- Reactivity: Reacts with human, African green monkey, dog, mouse, and rat samples .
Applications
The Mouse Anti-Human Hsp70 antibody is suitable for several applications:
- Immunohistochemistry (IHC-P): Used for detecting Hsp70 in tissue sections.
- Immunoprecipitation (IP): Helps in isolating Hsp70 protein from cell lysates.
- Flow Cytometry (Flow Cyt): Used for analyzing Hsp70 expression in cells.
- Western Blotting (WB): Detects Hsp70 protein in protein extracts.
- Immunocytochemistry/Immunofluorescence (ICC/IF): Visualizes Hsp70 in cultured cells .
The antibody’s specificity and versatility make it a valuable tool for studying the role of Hsp70 in various biological processes and diseases.
