HSP27 Antibody

Heat Shock Protein 27 , Mouse Anti Human
Cat. No.
BT11055
Source
Synonyms
HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
HSP27, also known as the Estrogen-Regulated 24K protein and hsp 28, belongs to the mammalian small heat shock protein family. It is constitutively expressed in many tissues, and its expression significantly increases in response to various stressors such as high temperatures, toxic metals, drugs, and oxidants. HSP27 is phosphorylated in vivo at three specific sites (Ser15, Ser78, and Ser82) by protein kinases, including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
1 mg/ml in PBS, pH 7.4, with 0.1% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
HSP27 antibody has been validated for ELISA, Western blot, and immunohistochemistry, ensuring its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. The recommended dilution range for Western blotting is 1:1,000-1:2,000, with a starting dilution of 1:1,000. For immunohistochemistry, the recommended dilution range is 1:50-1:100, with a starting dilution of 1:50.
Synonyms
HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Purification Method
HSP27 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P2A5AT.
Immunogen
Anti-human HSP27 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP27 amino acids 1-205 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Mouse Anti-Human Antibodies

Overview

Mouse anti-human antibodies are monoclonal antibodies produced in mice that are specific to human antigens. These antibodies are widely used in research, diagnostics, and therapeutic applications due to their high specificity and affinity for human targets .

Production and Applications

Mouse anti-human antibodies are generated by immunizing mice with human antigens, followed by the isolation and cloning of antibody-producing B cells. These antibodies can be used in various applications, including:

  • Diagnostics: Detection of specific human proteins or pathogens in clinical samples.
  • Research: Studying human proteins, cells, and tissues in laboratory settings.
  • Therapeutics: Treatment of diseases by targeting specific human antigens, such as cancer cells or inflammatory molecules .

Human Anti-Mouse Antibody (HAMA) Response

One challenge with using mouse-derived antibodies in humans is the potential for the Human Anti-Mouse Antibody (HAMA) response. This immune response occurs when the human body recognizes the mouse antibodies as foreign and produces antibodies against them. The HAMA response can range from mild allergic reactions to severe complications and can reduce the efficacy of the treatment .

Advances in Antibody Engineering

To overcome the limitations of the HAMA response, advances in antibody engineering have led to the development of chimeric, humanized, and fully human antibodies. These engineered antibodies have reduced immunogenicity and improved therapeutic potential .

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Heat Shock Protein 27 Human Recombinant
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