HS3ST1 Human
h3-OST-1, HS3ST1, 3OST, 3OST1, HS3S1.
Description
HS3ST1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
The product is provided as a solution of HS3ST1 protein at a concentration of 0.25 milligrams per milliliter. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and also contains 0.2M NaCl, 40% glycerol, and 2mM DTT.
h3-OST-1, HS3ST1, 3OST, 3OST1, HS3S1.
Product Science Overview
Heparan Sulfate 3-O-Sulfotransferase 1 (HS3ST1) is an enzyme that plays a crucial role in the biosynthesis of heparan sulfate, a linear polysaccharide found in the extracellular matrix and on cell surfaces. This enzyme is responsible for the transfer of a sulfo group to the 3-position of glucosamine residues in heparan sulfate, a modification that is essential for the biological activity of heparan sulfate .
HS3ST1 is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity and anticoagulant heparan sulfate conversion activity . The enzyme uses 3’-phospho-5’-adenylyl sulfate (PAPS) as a sulfo group donor to catalyze the transfer of the sulfo group . This reaction is considered the rate-limiting step in the biosynthesis of anticoagulant heparan sulfate .
Heparan sulfate proteoglycans (HSPGs) are involved in various physiological processes, including embryogenesis, angiogenesis, blood coagulation, and inflammation . The 3-O-sulfation of heparan sulfate by HS3ST1 is a rare but critical modification that influences the binding of heparan sulfate to a variety of proteins, including growth factors, cytokines, and extracellular matrix components . These interactions can affect cellular processes such as proliferation, migration, apoptosis, and immune response .
Alterations in the expression of HS3ST1 have been observed in various cancers . Dysregulation of this enzyme can lead to significant changes in the structure of heparan sulfate, impacting tumorigenesis and cancer progression . The enzyme’s role in modifying heparan sulfate also makes it a potential target for therapeutic strategies in cancer treatment .
Recombinant human HS3ST1 is produced using in vitro expression systems, such as wheat germ expression systems . The recombinant protein typically includes a GST tag at the N-terminal and corresponds to the amino acids 1-307 of the full-length human HS3ST1 . This recombinant form is used in various research applications, including Western blotting, ELISA, and protein array .
