MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL.
HRSP12 plays a crucial role in inhibiting protein translation by cleaving messenger RNA (mRNA). It specifically cleaves phosphodiester bonds in single-stranded RNA, thereby inhibiting cell-free protein synthesis . This mechanism is vital for regulating protein synthesis within cells and maintaining cellular homeostasis.
The levels of HRSP12 are significantly reduced in hepatocellular tumors and human hepatoma cell lines compared to normal liver tissues . This reduction in HRSP12 levels has led to the suggestion that it may serve as an important biomarker for hepatic carcinoma . The protein’s expression levels vary depending on the grade of the tumor, indicating its potential role in cancer diagnosis and prognosis .
Recombinant HRSP12 is typically produced in Escherichia coli (E. coli) expression systems. The recombinant protein is often tagged with a His-tag to facilitate purification. The molecular mass of the recombinant HRSP12 is approximately 16.7 kDa, and it is supplied as a 0.2 μm filtered solution containing 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, and 10% glycerol at pH 8.0 .