HPGDS Human

Hematopoietic Prostaglandin D Synthase Human Recombinant
Cat. No.
BT24190
Source
Escherichia Coli.
Synonyms
Hematopoietic prostaglandin D synthase, H-PGDS, GST class-sigma, Glutathione S-transferase, Glutathione-dependent PGD synthase, Glutathione-requiring prostaglandin D synthase, Prostaglandin-H2 D-isomerase, HPGDS, GSTS, PGDS, PTGDS2GSTS, PGD2, GSTS1-1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HPGDS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-199 a.a.) and having a molecular mass of 25.9kDa.
HPGDS is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Hematopoietic Prostaglandin D Synthase (HPGDS) is a member of the sigma class glutathione-S-transferase family. It catalyzes the conversion of PGH2 to PGD2, playing a crucial role in prostanoid production within the immune system and mast cells. HPGDS serves as a marker for human megakaryocyte differentiation stages. Additionally, this prostaglandin is involved in the regulation of smooth muscle contraction and relaxation, acts as a potent inhibitor of platelet aggregation, and facilitates the conjugation of glutathione with various aryl halides and organic isothiocyanates. HPGDS exhibits minimal glutathione-peroxidase activity towards cumene hydroperoxide. While expressed in several megakaryocytic cell lines, it is absent in platelets. High HPGDS expression is observed in adipose tissue, macrophages, and the placenta, while lower levels are detected in the lung, heart, lymph nodes, appendix, bone marrow, and fetal liver.
Description
Recombinant human HPGDS, expressed in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 25.9 kDa. It consists of 223 amino acids, comprising the HPGDS sequence (amino acids 1-199) and a 24 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The HPGDS protein solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, 0.15 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for extended storage durations. Repeated freezing and thawing should be avoided.
Purity
The purity of the HPGDS protein is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
Hematopoietic prostaglandin D synthase, H-PGDS, GST class-sigma, Glutathione S-transferase, Glutathione-dependent PGD synthase, Glutathione-requiring prostaglandin D synthase, Prostaglandin-H2 D-isomerase, HPGDS, GSTS, PGDS, PTGDS2GSTS, PGD2, GSTS1-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMPNYKL TYFNMRGRAE IIRYIFAYLD IQYEDHRIEQ ADWPEIKSTL PFGKIPILEV DGLTLHQSLA IARYLTKNTD LAGNTEMEQC HVDAIVDTLD DFMSCFPWAE KKQDVKEQMF NELLTYNAPH LMQDLDTYLG GREWLIGNSV TWADFYWEIC
STTLLVFKPD LLDNHPRLVT LRKKVQAIPA VANWIKRRPQ TKL.

Product Science Overview

Introduction

Hematopoietic Prostaglandin D Synthase (HPGDS) is an enzyme that plays a crucial role in the production of prostaglandin D2 (PGD2), a bioactive lipid involved in various physiological processes such as inflammation, allergic reactions, and sleep regulation . This enzyme is a member of the sigma class of glutathione S-transferases (GSTs) and is also known as glutathione-dependent PGD synthase .

Structure and Expression

HPGDS is a human full-length protein, typically expressed in Escherichia coli for recombinant production . The recombinant form of HPGDS is often used in research and has a purity level of over 95%, making it suitable for various applications such as SDS-PAGE and HPLC . The enzyme consists of 199 amino acids and has a molecular mass of approximately 24-26 kDa under reducing conditions .

Function and Mechanism

HPGDS catalyzes the conversion of prostaglandin H2 (PGH2) to PGD2, a major prostanoid produced in various tissues . This reaction is glutathione-dependent, meaning that the enzyme requires reduced glutathione (GSH) to function. The specific activity of recombinant HPGDS has been measured to be over 650 pmol/min/μg under specific conditions .

Biological Significance

PGD2, the product of the reaction catalyzed by HPGDS, is involved in several important physiological processes. It plays a significant role in mediating inflammation and allergic responses, and it is also implicated in the regulation of sleep . Due to its involvement in these processes, HPGDS has been a target for developing inhibitors as potential anti-inflammatory agents .

Applications in Research

Recombinant HPGDS is widely used in biochemical and pharmacological research. It is employed in studies aimed at understanding the enzyme’s role in various physiological and pathological conditions. Additionally, it is used in the development of inhibitors that could serve as therapeutic agents for conditions such as asthma, allergic rhinitis, and other inflammatory diseases .

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