HPCAL1 Human

Hippocalcin-Like 1 Human Recombinant
Cat. No.
BT25805
Source
Escherichia Coli.
Synonyms
Hippocalcin-Like 1, HLP2, BDR1, Calcium-Binding protein BDR-1, Visinin-Like protein 3,
VILIP-3
Appearance

Sterile filtered colorless solution.

Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HPCAL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213amino acids (1-193a.a.) and having a molecular wieght of 24.4kDa. The HPCAL1 is fused to 20a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
HPCAL1, a member of the neuron-specific calcium-binding protein family, is located in the retina and brain. It shares significant similarity with human hippocalcin protein and is nearly identical to rat and mouse hippocalcin-like 1 proteins. HPCAL1 plays a role in the calcium-dependent regulation of rhodopsin phosphorylation and may be involved in neuronal signaling within the central nervous system.
Description
Recombinant human HPCAL1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 213 amino acids (1-193a.a.), with a molecular weight of 24.4 kDa. A 20a.a. His-Tag is fused to the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The HPCAL1 protein solution is provided at a concentration of 1mg/ml in a 20mM Tris-HCl buffer (pH 8.0). The buffer also contains 1mM DTT, 0.2M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeding 95.0% as assessed by SDS-PAGE.
Synonyms
Hippocalcin-Like 1, HLP2, BDR1, Calcium-Binding protein BDR-1, Visinin-Like protein 3,
VILIP-3
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF

Product Science Overview

Introduction

Hippocalcin-like protein 1 (HPCAL1) is a member of the neuronal calcium sensor protein family. This protein is highly similar to human hippocalcin and nearly identical to rat and mouse hippocalcin-like 1 proteins . It plays a crucial role in the calcium-dependent regulation of rhodopsin phosphorylation and is significant in neuronal signaling within the central nervous system .

Gene and Protein Structure

The HPCAL1 gene is located on chromosome 2 in humans (2p25.1) and chromosome 12 in mice . The gene produces two alternatively spliced transcript variants with multiple polyadenylation sites . The protein encoded by this gene is involved in various cellular processes, particularly those related to calcium signaling.

Biological Functions

HPCAL1 is involved in the regulation of several cellular processes:

  • Calcium-Dependent Regulation: HPCAL1 regulates rhodopsin phosphorylation in a calcium-dependent manner, which is essential for proper neuronal signaling .
  • Neuronal Signaling: It is significant in the central nervous system, contributing to various neuronal functions .
Role in Cancer

Recent studies have highlighted the role of HPCAL1 in cancer, particularly non-small cell lung carcinoma (NSCLC). HPCAL1 has been reported to regulate cancer growth by activating LDHA (Lactate Dehydrogenase A), which promotes the growth of NSCLC . Knockdown of HPCAL1 has shown to inhibit NSCLC cell growth and tumor growth in vivo .

Research and Clinical Implications

The discovery of HPCAL1’s role in cancer opens new avenues for research and potential therapeutic interventions. By targeting HPCAL1, it may be possible to develop new treatments for cancers where this protein plays a critical role.

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