HNMT Human, Active
HMT, HNMT-S1, HNMT-S2, MRT51.
Greater than 90.0% as determined by SDS-PAGE.
Description
HNMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 328 amino acids (1-292 a.a) and having a molecular mass of 37.4kDa. HNMT is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Histamine N-Methyltransferase (HNMT) is a cytosolic enzyme that utilizes S-adenosyl-L-methionine as a methyl donor to inactivate histamine. This inactivation plays a role in regulating the immune system's response. HNMT is primarily active in tissues like the kidneys, central nervous system, and bronchi. It is essential for histamine degradation, regulation, and the airway's response to histamine.
Recombinant human HNMT, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 328 amino acids (with the mature protein encompassing residues 1-292) and has a molecular weight of 37.4 kDa. The protein includes a 36 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
The HNMT protein solution has a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It's important to prevent repeated freeze-thaw cycles.
The specific activity is measured to be greater than 200 nmol/min/mg. This indicates the enzyme's efficiency and is determined by the amount of enzyme required to transfer 1.0 nmole of a methyl group per minute at a temperature of 37°C.
Analysis by SDS-PAGE confirms a purity level exceeding 90.0%.
HMT, HNMT-S1, HNMT-S2, MRT51.
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA.
Product Science Overview
Histamine N-Methyltransferase (HNMT) is an enzyme that plays a crucial role in the metabolism of histamine, a biogenic amine involved in various physiological processes such as immune response, gastric acid secretion, and neurotransmission . The recombinant form of this enzyme, produced through genetic engineering techniques, is used in research and therapeutic applications.
HNMT is a small monomeric protein consisting of 292 amino acid residues and has a molecular weight of approximately 33 kDa . It catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to the secondary amino group of the imidazole ring of histamine, forming Nτ-methylhistamine . This reaction is essential for the inactivation of histamine, particularly in the central nervous system (CNS), where it regulates histaminergic neurotransmission .
Histamine is involved in a wide range of physiological functions, including:
- Immune Response: Histamine modulates the immune response through interaction with four distinct receptor subtypes (H1, H2, H3, and H4) .
- Gastric Acid Secretion: It stimulates the secretion of gastric acid in the stomach.
- Neurotransmission: In the CNS, histamine acts as a neurotransmitter, influencing various brain functions .
HNMT, along with diamine oxidase (DAO), is one of the two primary enzymes responsible for histamine metabolism in mammals . Unlike DAO, which is found in peripheral tissues, HNMT is uniquely present in the CNS .
Alterations in HNMT activity have been associated with several neurological and psychiatric disorders. For instance, single nucleotide polymorphisms (SNPs) in the HNMT gene have been linked to conditions such as Parkinson’s disease and attention deficit hyperactivity disorder (ADHD) . Additionally, postmortem studies have shown altered HNMT expression in various brain diseases .
The recombinant form of HNMT is produced using genetic engineering techniques, where the HNMT gene is cloned and expressed in suitable host cells. This recombinant enzyme is used in research to study histamine metabolism and its role in various physiological and pathological processes. It is also being explored for potential therapeutic applications, particularly in conditions where histamine regulation is disrupted .
