HMGCS1 Human

3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 Human Recombinant

Cat. No.

BT24123

Source

Escherichia Coli.

Synonyms

3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble), 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1 (Soluble), 3-Hydroxy-3-Methylglutaryl Coenzyme A (HMG-CoA) Synthase, EC 2.3.3.10, HMGCS, Hydroxymethylglutaryl-CoA Synthase, Cytoplasmic, 3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase, HMG-CoA Synthase.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

HMGCS1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 543 amino acids (1-520 a.a) and having a molecular mass of 59.7kDa.
HMGCS1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

3-Hydroxy-3-Methylglutaryl-CoA Synthase 1, also known as HMGCS1, is a member of the HMG-CoA synthase family. This enzyme catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA to form HMG-CoA, a key substrate for HMG-CoA reductase.

Description

Recombinant human HMGCS1, expressed in E. coli, is a non-glycosylated polypeptide chain containing 543 amino acids (residues 1-520) with a molecular weight of 59.7 kDa. The protein includes an N-terminal His-tag of 23 amino acids and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, sterile-filtered solution.

Formulation

The HMGCS1 protein is supplied at a concentration of 0.5 mg/ml in a solution containing phosphate buffered saline (pH 7.4), 10% glycerol, and 1 mM DTT.

Stability

For short-term storage (2-4 weeks), the protein can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

The purity of the HMGCS1 protein is greater than 80% as determined by SDS-PAGE.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPGSLPL NAEACWPKDV GIVALEIYFP SQYVDQAELE KYDGVDAGKY TIGLGQAKMG FCTDREDINS LCMTVVQNLM ERNNLSYDCI GRLEVGTETI IDKSKSVKTN LMQLFEESGN TDIEGIDTTN ACYGGTAAVF NAVNWIESSS WDGRYALVVA GDIAVYATGN ARPTGGVGAV ALLIGPNAPL IFERGLRGTH MQHAYDFYKP DMLSEYPIVD GKLSIQCYLS ALDRCYSVYC KKIHAQWQKE GNDKDFTLND FGFMIFHSPY CKLVQKSLAR MLLNDFLNDQ NRDKNSIYSG LEAFGDVKLE DTYFDRDVEK AFMKASSELF SQKTKASLLV SNQNGNMYTS SVYGSLASVL AQYSPQQLAG KRIGVFSYGS GLAATLYSLK VTQDATPGSA LDKITASLCD LKSRLDSRTG VAPDVFAENM KLREDTHHLV NYIPQGSIDS LFEGTWYLVR VDEKHRRTYA RRPTPNDDTL DEGVGLVHSN IATEHIPSPA KKVPRLPATA AEPEAAVISN GEH

Product Science Overview

Introduction

3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (HMGCS1) is a crucial enzyme in the mevalonate pathway, which is essential for the biosynthesis of cholesterol and other isoprenoids. This enzyme catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-Hydroxy-3-Methylglutaryl-CoA (HMG-CoA), a key intermediate in the pathway.

Gene and Protein Structure

The HMGCS1 gene is located on chromosome 5 in humans and encodes a protein that is approximately 520 amino acids in length. The enzyme is predominantly found in the cytoplasm and is known to form homodimers, which are essential for its catalytic activity.

Function and Mechanism

HMGCS1 plays a pivotal role in the mevalonate pathway by catalyzing the first committed step in the synthesis of HMG-CoA. This reaction involves the condensation of acetyl-CoA and acetoacetyl-CoA, which is then converted by HMG-CoA reductase (HMGCR) into mevalonate. Mevalonate is a precursor for the synthesis of cholesterol, steroid hormones, and other essential biomolecules.

Clinical Significance

Mutations or dysregulation of the HMGCS1 gene can lead to various metabolic disorders. For instance, congenital myopathy with rigid spine and cardiac sarcoidosis have been associated with abnormalities in the HMGCS1 gene. Additionally, the enzyme’s role in cholesterol biosynthesis makes it a potential target for therapeutic interventions aimed at lowering cholesterol levels.

Recombinant HMGCS1

Recombinant HMGCS1 is produced using recombinant DNA technology, which involves cloning the HMGCS1 gene into an expression vector and introducing it into a host organism, such as Escherichia coli, for protein production. The recombinant enzyme is then purified and used for various research and therapeutic applications.

Research and Applications

Recombinant HMGCS1 is widely used in biochemical research to study the mevalonate pathway and its regulation. It is also employed in drug discovery efforts aimed at identifying inhibitors of HMGCS1 as potential cholesterol-lowering agents. Furthermore, the enzyme is used in structural biology studies to elucidate its three-dimensional structure and understand the molecular basis of its catalytic activity.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

HMGCS1 Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service