HIV-1 p30
Escherichia Coli.
Sterile Filtered clear solution.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
Recombinant HIV-1 p30 produced in E. coli having a Mw of 30kDa. Recombinant HIV-1 p30 is fused to a 6xHis tag at its C-terminus and purified by proprietary chromatographic technique.
Product Specs
Escherichia Coli.
Product Science Overview
HIV-1 p30 is a recombinant protein derived from the Human Immunodeficiency Virus type 1 (HIV-1). This protein is a part of the Gag polyprotein, which plays a crucial role in the assembly and maturation of the virus. The recombinant form of HIV-1 p30 is often used in research and vaccine development due to its immunogenic properties.
The HIV-1 p30 protein is a segment of the larger Gag polyprotein, which also includes other important proteins such as p24, p17, and p7. The Gag polyprotein is essential for the formation of the viral core, which houses the viral RNA and enzymes necessary for replication. The p30 protein specifically contributes to the structural integrity of the virus and is involved in the assembly of the viral capsid.
Recombinant HIV-1 p30 is produced using various expression systems, such as bacterial, yeast, or mammalian cells. The gene encoding the p30 protein is cloned into an expression vector, which is then introduced into the host cells. These cells are cultured under conditions that promote the expression of the recombinant protein. The protein is subsequently purified using techniques like affinity chromatography.
- Vaccine Development: Recombinant HIV-1 p30 is used as an antigen in vaccine formulations. Its ability to elicit an immune response makes it a valuable component in the development of HIV vaccines.
- Diagnostic Tools: The protein is also used in diagnostic assays to detect HIV infection. Enzyme-linked immunosorbent assays (ELISAs) often utilize recombinant p30 to capture antibodies from patient samples.
- Immunological Studies: Researchers use recombinant p30 to study the immune response to HIV infection. This helps in understanding how the virus evades the immune system and in developing strategies to counteract it.
