GCK Human, Active
Glucokinase, Glucokinase (Hexokinase 4), Hexokinase Type IV, HK IV, HK4, Maturity Onset Diabetes of The Young 2, ATP:D-Hexose 6-Phosphotransferase, Hexokinase D, Pancreatic Isozyme, Hexokinase-4, Hexokinase-D, Hexokinase 4.
Description
GCK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 485 amino acids (1-465 a.a) and having a molecular mass of 54.3kDa. GCK is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Glucokinase, Glucokinase (Hexokinase 4), Hexokinase Type IV, HK IV, HK4, Maturity Onset Diabetes of The Young 2, ATP:D-Hexose 6-Phosphotransferase, Hexokinase D, Pancreatic Isozyme, Hexokinase-4, Hexokinase-D, Hexokinase 4.
MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ.
Product Science Overview
Hexokinase-4, also known as Glucokinase (GCK), is an enzyme that plays a crucial role in the regulation of carbohydrate metabolism. It is primarily found in the liver and pancreatic beta cells of humans and other vertebrates. This enzyme is responsible for the phosphorylation of glucose to produce glucose-6-phosphate, a key step in the glycolytic pathway .
Hexokinase-4 (Human Recombinant, Active) is produced using recombinant DNA technology. The human GCK gene is cloned and expressed in Escherichia coli (E. coli) to produce a single, non-glycosylated polypeptide chain containing 485 amino acids. This recombinant protein has a molecular mass of approximately 54.3 kDa and includes a 20 amino acid His-tag at the N-terminus for purification purposes .
Hexokinase-4 differs from other hexokinases in its kinetic properties and regulatory mechanisms. Unlike other hexokinases, which have a high affinity for glucose, Hexokinase-4 has a lower affinity, allowing it to act as a glucose sensor. This property is particularly important in the liver and pancreatic beta cells, where it helps regulate blood glucose levels. When glucose levels are high, Hexokinase-4 facilitates the conversion of glucose to glucose-6-phosphate, which can then enter various metabolic pathways .
Hexokinase-4 (Human Recombinant, Active) is typically stored at 4°C if used within 2-4 weeks. For longer storage, it is recommended to keep the enzyme frozen at -20°C, with the addition of a carrier protein such as 0.1% Human Serum Albumin (HSA) or Bovine Serum Albumin (BSA) to enhance stability. It is important to avoid multiple freeze-thaw cycles to maintain enzyme activity .
