HEXA Human, Sf9

Hexosaminidase A Human Recombinant, SF9
Cat. No.
BT24872
Source
Sf9, Baculovirus cells.
Synonyms
Hexosaminidase A (Alpha Polypeptide), N-Acetyl-Beta-Glucosaminidase Subunit Alpha, Beta-N-Acetylhexosaminidase Subunit Alpha, Hexosaminidase Subunit A, EC 3.2.1.52, TSD, Beta-Hexosaminidase Subunit Alpha, GM2 Gangliosidosis, Tay Sachs Disease, EC 3.2.1, Beta-hexosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, Hexosaminidase subunit A, N-acetyl-beta-glucosaminidase subunit alpha.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HEXA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 513 amino acids (23-529a.a.) and having a molecular mass of 59.2kDa. 
(Molecular size on SDS-PAGE will appear at approximately 50-70kDa). 
HEXA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
HEXA, the alpha subunit of the lysosomal enzyme beta-hexosaminidase, plays a crucial role in the breakdown of ganglioside GM2 and other molecules with N-acetyl hexosamines terminus, working in conjunction with the GM2 activator protein. Beta-hexosaminidase consists of alpha and beta subunits, each encoded by different genes and belonging to the glycosyl hydrolases family. Mutations in the alpha subunit gene can lead to Tay-Sachs disease (GM2-gangliosidosis type I).
Description
Produced in Sf9 Baculovirus cells, HEXA is a single, glycosylated polypeptide chain with a molecular mass of 59.2 kDa. It comprises 513 amino acids (23-529a.a.). On SDS-PAGE, the molecular size appears between 50-70 kDa. HEXA is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The HEXA protein solution (0.5 mg/ml) is prepared in phosphate buffered saline with a pH of 7.4.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity level exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Hexosaminidase A (Alpha Polypeptide), N-Acetyl-Beta-Glucosaminidase Subunit Alpha, Beta-N-Acetylhexosaminidase Subunit Alpha, Hexosaminidase Subunit A, EC 3.2.1.52, TSD, Beta-Hexosaminidase Subunit Alpha, GM2 Gangliosidosis, Tay Sachs Disease, EC 3.2.1, Beta-hexosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, Hexosaminidase subunit A, N-acetyl-beta-glucosaminidase subunit alpha.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
LWPWPQNFQT SDQRYVLYPN NFQFQYDVSS AAQPGCSVLD EAFQRYRDLL FGSGSWPRPY LTGKRHTLEK NVLVVSVVTP GCNQLPTLES VENYTLTIND DQCLLLSETV WGALRGLETF SQLVWKSAEG TFFINKTEIE DFPRFPHRGL LLDTSRHYLP LSSILDTLDV MAYNKLNVFH WHLVDDPSFP YESFTFPELM RKGSYNPVTH IYTAQDVKEV IEYARLRGIR VLAEFDTPGH TLSWGPGIPG LLTPCYSGSE PSGTFGPVNP SLNNTYEFMS TFFLEVSSVF PDFYLHLGGD EVDFTCWKSN PEIQDFMRKK GFGEDFKQLE SFYIQTLLDI VSSYGKGYVV WQEVFDNKVK IQPDTIIQVW REDIPVNYMK ELELVTKAGF RALLSAPWYL NRISYGPDWK DFYIVEPLAF EGTPEQKALV IGGEACMWGE YVDNTNLVPR LWPRAGAVAE RLWSNKLTSD LTFAYERLSH FRCELLRRGV QAQPLNVGFC EQEFEQTHHH HHH.

Product Science Overview

Recombinant Production

Hexosaminidase A (Human Recombinant, SF9) refers to the recombinant form of the human Hexosaminidase A enzyme produced using the Sf9 insect cell expression system. Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used in biotechnology for the production of recombinant proteins due to their high efficiency and ability to perform post-translational modifications similar to those in human cells .

Structure and Function

The recombinant Hexosaminidase A enzyme produced in Sf9 cells retains the same structural and functional properties as the naturally occurring enzyme. It hydrolyzes the terminal N-acetyl-D-hexosamine residues in various glycoconjugates, including oligosaccharides, glycolipids, and mucopolysaccharides . This hydrolytic activity is essential for the breakdown and recycling of these complex molecules within lysosomes.

Isoenzymes and Specificity

Hexosaminidase A is one of the two major isoenzymes of lysosomal β-N-acetyl hexosaminidase, the other being Hexosaminidase B (HexB). While HexA can hydrolyze both neutral and anionic substrates, HexB is more specific to neutral oligosaccharides . The unique ability of HexA to degrade GM2 gangliosides in the presence of the GM2 activator protein (GM2A) highlights its critical role in cellular metabolism .

Applications in Research

Recombinant Hexosaminidase A produced in Sf9 cells is widely used in research to study the enzyme’s structure, function, and role in diseases such as Tay-Sachs. It also serves as a valuable tool for developing therapeutic strategies aimed at correcting enzyme deficiencies .

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