ErbB3 Human
Tyrosine Kinase ErbB-3 Human Recombinant
Cat. No.
BT16160
Source
Escherichia Coli.
Synonyms
Receptor tyrosine-protein kinase erbB-3, EC 2.7.10.1, c-erbB3, Tyrosine kinase-type cell surface receptor HER3, ErbB3, HER3.
Appearance
A white semitransparent suspension at a concentration of 1 mg/ml.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Tyrosine Kinase ErbB3 Human Recombinant (HER3) produced in E.Coli is a single, non-glycosylated polypeptide consisting of several epitopes of extracellular domain of human Erb-b3, and having a total molecular mass of approximately 12.0 kDa.
The ErbB3 is purified by proprietary chromatographic techniques.
The ErbB3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
ErbB3, also known as HER3 (human epidermal growth factor receptor 3), belongs to the ErbB family of tyrosine kinase receptors. This family is known for its role in receiving signals from epidermal growth factor (EGF) family growth factors. Uniquely, ErbB3 possesses a kinase domain that is defective. This receptor is found in various cells, including keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts, and Schwann cells. While ErbB3 alone acts as a low-affinity receptor for heregulins (HRG), it can trigger a specific antibody response in living organisms, potentially suppressing tumor cell growth. ErbB-3 shows promise in treating breast cancer at various stages (early, medium, advanced, or post-operative) where ErbB2 is overexpressed. Due to its mode of action, ErbB3 is categorized as a therapeutic agent for cancer.
Description
Recombinant Human Tyrosine Kinase ErbB3 (HER3) is produced in E. coli. This non-glycosylated polypeptide is a single chain comprising several epitopes from the extracellular domain of human Erb-b3. Its total molecular weight is approximately 12.0 kDa. The purification of ErbB3 is achieved using specialized chromatographic techniques.
Physical Appearance
White, semi-transparent suspension with a concentration of 1 mg/ml.
Formulation
Each milligram of protein contains: 1 mg aluminum hydroxide, 10 mM arginine, 10 mM sodium chloride, 20 mM sodium phosphate buffer, and 5 mM potassium phosphate. To create a stock solution, it is recommended to dilute the contents of the vial with sterile phosphate-buffered saline containing 1 mg aluminum hydroxide.
Stability
ErbB3 remains stable at 4°C for one week. However, for optimal storage, it is recommended to keep it below -18°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freezing and thawing.
Purity
Exceeds 95.0% purity, as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis. (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
Evaluated based on its ability to delay tumor onset in FVB/N transgenic mice predisposed to spontaneous breast cancer. It effectively inhibits both the development of these tumors and the growth of in situ transplanted breast cancer in the same mouse model.
Synonyms
Receptor tyrosine-protein kinase erbB-3, EC 2.7.10.1, c-erbB3, Tyrosine kinase-type cell surface receptor HER3, ErbB3, HER3.
Source
Escherichia Coli.
Product Science Overview
Introduction
Gene and Expression
The ERBB3 gene is located on the long arm of chromosome 12 (12q13) and spans 23,651 base pairs, translating into 1342 amino acids . During human development, ERBB3 is expressed in various tissues including skin, bone, muscle, nervous system, heart, lungs, and intestinal epithelium . In adults, it is expressed in the gastrointestinal tract, reproductive system, skin, nervous system, urinary tract, and endocrine system .
Structure
ErbB-3 consists of three main domains:
- Extracellular Domain: Contains four subdomains (I-IV). Subdomains I and III are leucine-rich and primarily involved in ligand binding, while subdomains II and IV are cysteine-rich and contribute to protein conformation and stability through disulfide bonds .
- Transmembrane Domain: Anchors the protein in the cell membrane.
- Intracellular Domain: Contains a juxtamembrane segment, a kinase domain, and a C-terminal domain . The kinase domain in ErbB-3 is defective, making it unique among ErbB family members .
Function
ErbB-3 binds ligands such as heregulin and NRG-2, causing a conformational change that allows for dimerization, phosphorylation, and activation of signal transduction pathways . It can heterodimerize with other ErbB family members, leading to the activation of pathways that promote cell proliferation and differentiation . However, the theoretical ErbB-3 homodimer would be non-functional due to its kinase-impaired nature .
Recombinant ErbB-3
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