HDDC3 Human
HD domain containing 3 Human Recombinant
Cat. No.
BT28273
Source
Escherichia Coli.
Synonyms
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD domain-containing protein 3, Metazoan SpoT homolog 1, MESH1, Penta-phosphate guanosine-3'-pyrophosphohydrolase, (ppGpp)ase, HDDC3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
HDDC3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (1-140) and having a molecular mass of 17.9kDa.
HDDC3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
HDDC3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1 (HDDC3) is an enzyme that plays a crucial role in bacterial metabolism by regulating the levels of the signaling molecule ppGpp. HDDC3 possesses an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for manganese ion binding, which are essential for its catalytic activity. This enzyme effectively catalyzes the hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo, effectively reducing its cellular concentration. Furthermore, HDDC3 can compensate for the loss of SpoT function, a key enzyme involved in ppGpp synthesis, and mitigate the growth inhibitory effects caused by RelA-mediated ppGpp accumulation in bacteria.
Description
Recombinant human HDDC3, expressed in E. coli, is a purified protein with a molecular weight of 17.9 kDa. It consists of a single polypeptide chain of 160 amino acids, with the first 140 amino acids representing the HDDC3 protein and a 20 amino acid His-tag fused at the N-terminus to facilitate purification. The protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear, colorless solution without any visible particles.
Formulation
The HDDC3 protein is supplied in a solution containing 20mM Tris-HCl buffer at pH 8.0, 40% glycerol, 0.15M NaCl, and 1mM DTT. The protein concentration is 0.5mg/ml.
Stability
For short-term storage (up to 4 weeks), keep the HDDC3 vial refrigerated at 4°C. For long-term storage, freeze the vial at -20°C. It is highly recommended to add a carrier protein such as HSA or BSA at a final concentration of 0.1% to prevent protein degradation during long-term storage. Avoid repeated freezing and thawing of the protein solution.
Purity
The purity of HDDC3 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD domain-containing protein 3, Metazoan SpoT homolog 1, MESH1, Penta-phosphate guanosine-3'-pyrophosphohydrolase, (ppGpp)ase, HDDC3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ.
Product Science Overview
Gene and Protein Information
- Gene Symbol: HDDC3
- Aliases: MESH1, Guanosine-3’,5’-Bis (Diphosphate) 3’-Pyrophosphohydrolase, Metazoan SpoT Homolog 1 (PpGpp)Ase
- Chromosomal Location: Chromosome 15
- Protein Function: HDDC3 is a guanosine-3’,5’-bis (diphosphate) 3’-diphosphatase, which means it is involved in the hydrolysis of guanosine pentaphosphate (ppGpp), a molecule that plays a crucial role in the bacterial stringent response to nutrient deprivation .
Biological Role
Expression and Localization
Research and Clinical Significance
Research on HDDC3 has shown its involvement in several physiological and pathological processes:
- Ferroptosis: HDDC3 plays a role in ferroptosis by depleting NADPH in stressed cells, which can lead to cell death through oxidative stress .
- Metabolic Regulation: By hydrolyzing ppGpp, HDDC3 helps regulate metabolic processes during nutrient deprivation .
- Potential Therapeutic Target: Due to its role in stress responses and cell death, HDDC3 is being studied as a potential therapeutic target for diseases related to oxidative stress and metabolic dysregulation .
Experimental Data
Studies using mouse models have provided insights into the function and significance of HDDC3. Knockout mice for the Hddc3 gene have shown various phenotypic changes, including increased levels of circulating alanine and aspartate transaminases, abnormal pancreas and kidney morphology, and enlarged spleen and heart .
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