The HDDC2 gene is located on chromosome 6 and is also known by several alternate names, including C6orf74, CGI-130, and HCV NS5A-transactivated protein 2 . The gene encodes a protein that consists of 204 amino acids and has a predicted molecular mass of approximately 25.8 kDa . The recombinant form of this protein is often produced with an N-terminal His-tag to facilitate purification and detection .
Recombinant HD Domain Containing 2 is typically expressed in Escherichia coli (E. coli), a common bacterial host used for protein production . The protein is purified using affinity chromatography, which exploits the His-tag for efficient isolation. The purified protein is then subjected to various quality control measures, including SDS-PAGE, to ensure its purity and integrity .
The exact biological function of HD Domain Containing 2 is still under investigation. However, it is believed to play a role in cellular processes influenced by the HD domain. The HD domain is implicated in signal transduction pathways and may have enzymatic functions, although specific substrates and activities are yet to be fully elucidated .
Recombinant HD Domain Containing 2 is primarily used in research settings to study its structure and function. It serves as a valuable tool for investigating the role of HD domain-containing proteins in various cellular processes. Additionally, it can be used in assays to identify potential interacting partners and to explore its involvement in disease mechanisms .