HCST Human
Sf9, Baculovirus cells.
Hematopoietic Cell Signal Transducer, DNAX-Activation Protein 10, Phosphoinositide-3-Kinase Adaptor Protein, Transmembrane Adapter Protein KAP10, Kinase Assoc Pro Of ~10kDa, Membrane Protein DAP10, PIK3AP, DAP10, KAP10, Kinase Assoc Protein.
Greater than 90.0% as determined by SDS-PAGE.
Description
HCST produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 271 amino acids (20-48 a.a.) and having a molecular mass of 30.1kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
HCST is expressed with a 242 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Hematopoietic Cell Signal Transducer (HCST) belongs to the DAP10 family and is involved in forming immunoreceptor complexes. It plays a crucial role in initiating cytotoxic responses against MHC class I chain-associated molecules like MICA and target cells displaying surface ligands such as UL16-binding protein (ULBP) and MICB. The HCST complex contributes to cell proliferation and survival by activating T cell and natural killer (NK) cell responses.
Produced in Sf9 insect cells using a baculovirus expression system, HCST is a single, glycosylated polypeptide chain consisting of 271 amino acids (specifically, residues 20 to 48). It has a molecular weight of 30.1 kDa. On SDS-PAGE analysis, the apparent molecular size ranges from 28 to 40 kDa.
A 242 amino acid human IgG-His tag is fused to the C-terminus of HCST. The protein is purified using proprietary chromatographic techniques.
The HCST protein solution is provided at a concentration of 1 mg/ml. It is formulated in a solution containing 10% glycerol and Phosphate Buffered Saline at a pH of 7.4.
To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%.
Repeated freezing and thawing of the product should be avoided.
The purity of HCST is greater than 90% as determined by SDS-PAGE analysis.
Hematopoietic Cell Signal Transducer, DNAX-Activation Protein 10, Phosphoinositide-3-Kinase Adaptor Protein, Transmembrane Adapter Protein KAP10, Kinase Assoc Pro Of ~10kDa, Membrane Protein DAP10, PIK3AP, DAP10, KAP10, Kinase Assoc Protein.
Sf9, Baculovirus cells.
ADPTTPGERS SLPAFYPGTS GSCSGCGSLS LPLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
The Hematopoietic Cell Signal Transducer (HCST), also known as DNAX-activation protein 10 (DAP10), is a transmembrane signaling adaptor protein. It plays a crucial role in the immune system by participating in the activation of natural killer (NK) cells and certain subsets of T cells. This protein is encoded by the HCST gene in humans.
The HCST gene is located on chromosome 19 and encodes a protein that contains a YxxM motif in its cytoplasmic domain . This motif is essential for the activation of phosphatidylinositol 3-kinase (PI3K) dependent signaling pathways . The protein is a part of the DAP10 family and is capable of forming an immunoreceptor complex .
HCST is primarily involved in the immune recognition receptor complex with the C-type lectin-like receptor NKG2D . This receptor complex is crucial for the activation of NK and T cell responses. The ligands for this receptor include MHC class I chain-related proteins (MICA and MICB) and UL16-binding proteins (ULBPs), which are upregulated under stress conditions such as viral infections and tumor transformations .
When the NKG2D receptor binds to its ligands, HCST activates PI3K signaling pathways through its YxxM motif . This activation leads to the induction of cytotoxicity against target cells expressing these ligands . The HCST-NKG2D receptor complex plays a significant role in cell survival and proliferation by triggering cytotoxic responses .
Mutations or dysregulation of the HCST gene can be associated with various diseases. For instance, HCST has been linked to conditions such as intracranial berry aneurysms and peroxisome biogenesis disorders . Understanding the function and regulation of HCST is essential for developing therapeutic strategies targeting immune responses in diseases like cancer and viral infections.
Recombinant HCST is produced using genetic engineering techniques to express the HCST protein in a host system, such as bacteria or mammalian cells. This recombinant protein is used in research to study its structure, function, and role in immune signaling pathways. It is also utilized in developing therapeutic interventions aimed at modulating immune responses.
