HBQ1 Human

The HBQ1 gene is part of the human alpha-globin gene cluster, which includes five functional genes and two pseudogenes . Theta-globin mRNA is primarily found in human fetal erythroid tissue but is not present in adult erythroid or other non-erythroid tissues .

Hemoglobin Theta 1 consists of 142 amino acids and has a molecular mass of approximately 17.9 kDa . The recombinant form of this protein is often produced in E. coli and is fused to a His-tag at the N-terminus to facilitate purification . The protein’s structure allows it to bind oxygen with high affinity, which is essential for its role in oxygen transport.

Recombinant human HBQ1 is produced using conventional chromatography techniques. The protein is expressed in E. coli and purified to a high degree of purity, typically greater than 90% as determined by SDS-PAGE . The recombinant protein is often used in research to study hemoglobin function and structure.

Recombinant HBQ1 is used in various research applications, including studies on hemoglobin function, oxygen binding, and the effects of mutations on hemoglobin structure and function. It is also used in the development of therapeutic agents and in the study of hemoglobin-related diseases.

Recombinant HBQ1 should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain protein integrity .