HBA2 Human

Hemoglobin, Alpha 2 Human Recombinant
Cat. No.
BT19828
Source
E.coli.
Synonyms
Hemoglobin alpha chain, hemoglobin alpha 2, hemoglobin subunit alpha, Alpha-globin, alpha-2 globin, HBH.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HBA2 Human Recombinant produced in E. coli is a single polypeptide chain containing 179 amino acids (1-142) and having a molecular mass of 19.5 kDa.
HBA2 is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
HBA2, a member of the globin family, plays a crucial role in oxygen transportation from the lungs to various tissues. Although the coding sequences of HBA2 and HBA1 are identical, they exhibit minor differences in their 5' untranslated regions and introns, along with significant variations in their 3' untranslated regions. HbA, comprising two alpha and two beta chains, constitutes about 97% of adult hemoglobin. Alpha chains also combine with delta chains to form HbA-2, which, together with HbF (fetal hemoglobin), accounts for the remaining 3% of adult hemoglobin. Alpha thalassemia arises from the deletion of one or both alpha genes (HBA2 and HBA1), although non-deletion forms have also been observed.
Description
Recombinantly produced in E. coli, HBA2 Human is a single polypeptide chain weighing 19.5 kDa. This protein consists of 179 amino acids, with positions 1-142 representing the HBA2 sequence. A 37 amino acid His-tag is fused at the N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The HBA2 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 100mM NaCl, 2mM DTT, 2M urea, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C in a frozen state is recommended. The addition of a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity levels exceed 90% as determined by SDS-PAGE analysis.
Synonyms
Hemoglobin alpha chain, hemoglobin alpha 2, hemoglobin subunit alpha, Alpha-globin, alpha-2 globin, HBH.
Source
E.coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMVL SPADKTNVKA AWGKVGAHAG EYGAEALERM FLSFPTTKTY FPHFDLSHGS AQVKGHGKKV ADALTNAVAH VDDMPNALSA LSDLHAHKLR VDPVNFKLLS HCLLVTLAAH LPAEFTPAVH ASLDKFLASV STVLTSKYR

Product Science Overview

Introduction

Hemoglobin, Alpha 2 (HBA2) is a gene that encodes the alpha globin chain of hemoglobin in humans. Hemoglobin is a crucial protein in red blood cells responsible for transporting oxygen from the lungs to various tissues and organs throughout the body. The alpha globin chains, along with beta globin chains, form the most common type of hemoglobin in adults, known as Hemoglobin A (HbA). Hemoglobin A2 (HbA2) is a minor component of adult hemoglobin, consisting of two alpha and two delta chains, and constitutes about 2-3% of the total hemoglobin in adults .

Gene Structure and Function

The human alpha globin gene cluster is located on chromosome 16 and spans approximately 30 kilobases (kb). This cluster includes seven alpha-like globin genes and pseudogenes arranged in the following order: 5’- HBZ - HBZP1 - HBM - HBAP1 - HBA2 - HBA1 - HBQ1 -3’ . The HBA2 and HBA1 genes encode the alpha globin chains, which are identical in their coding sequences but differ slightly in their 5’ untranslated regions and introns. These genes play a vital role in the synthesis of hemoglobin, which is essential for oxygen transport in the body .

Recombinant Hemoglobin, Alpha 2

Recombinant Hemoglobin, Alpha 2 (Human Recombinant) is produced using recombinant DNA technology, which involves inserting the HBA2 gene into a suitable expression system, such as Escherichia coli (E. coli), to produce the protein in large quantities. This recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection . The recombinant HBA2 protein is used in various research applications, including studies on hemoglobin function, structure, and interactions with other molecules.

Clinical Significance

Mutations or deletions in the HBA2 gene can lead to alpha-thalassemia, a genetic disorder characterized by reduced or absent production of alpha globin chains. Alpha-thalassemia can result in various clinical manifestations, ranging from mild anemia to severe forms such as Hemoglobin H disease and hydrops fetalis . Understanding the structure and function of HBA2 is crucial for developing diagnostic and therapeutic approaches for these conditions.

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