HAUS1 Human

HAUS Augmin-Like Complex, Subunit 1 Human Recombinant

Cat. No.

BT14451

Source

Escherichia Coli.

Synonyms

HAUS augmin-like complex subunit 1, Coiled-coil domain-containing protein 5, Enhancer of invasion-cluster, HEI-C, HAUS1, CCDC5, HEIC, HsT1461.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

HAUS1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-278 a.a) and having a molecular mass of 34.4kDa.
HAUS1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

The human augmin complex, also known as the HAUS complex, is a multi-protein assembly consisting of eight subunits, including HAUS1. This complex plays a crucial role in microtubule generation within the mitotic spindle, which is essential for proper cell division. Specifically, HAUS1 contributes to mitotic spindle assembly, maintains centrosome integrity, and ensures the successful completion of cytokinesis. The expression of HAUS1 is widespread, with particularly high levels observed in the pancreas, kidney, skeletal muscle, liver, and heart. Conversely, its expression is relatively low in the lung, brain, and placenta. Depleting HAUS1 in cells does not disrupt functional cell cycle checkpoints; however, it does lead to a reduction in the G2/M cell cycle compartment and promotes apoptosis. Notably, the protein level of HAUS1 remains consistent throughout the cell cycle.

Description

Recombinant human HAUS1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 302 amino acids (with amino acids 1-278 being of interest) and possessing a molecular weight of 34.4 kDa. The protein includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution that has been sterilized by filtration.

Formulation

The HAUS1 protein solution has a concentration of 0.25 mg/ml and is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 50% glycerol, 0.2 M NaCl, and 2 mM DTT.

Stability

For optimal storage, the product should be kept at 4°C if the entire vial will be utilized within 2-4 weeks. For extended storage periods, freezing at -20°C is recommended. To further enhance long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advisable. It is important to avoid subjecting the product to repeated freeze-thaw cycles.

Purity

The purity of the HAUS1 protein is determined to be greater than 85.0% based on SDS-PAGE analysis.

Synonyms

HAUS augmin-like complex subunit 1, Coiled-coil domain-containing protein 5, Enhancer of invasion-cluster, HEI-C, HAUS1, CCDC5, HEIC, HsT1461.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMEPQEE RETQVAAWLK KIFGDHPIPQ YEVNPRTTEI LHHLSERNRV RDRDVYLVIE DLKQKASEYE SEAKYLQDLL MESVNFSPAN LSSTGSRYLN ALVDSAVALE TKDTSLASFI PAVNDLTSDL FRTKSKSEEI KIELEKLEKN LTATLVLEKC
LQEDVKKAEL HLSTERAKVD NRRQNMDFLK AKSEEFRFGI KAAEEQLSAR GMDASLSHQS LVALSEKLAR LKQQTIPLKK KLESYLDLMP NPSLAQVKIE EAKRELDSIE AELTRRVDMM EL.

Product Science Overview

Introduction

The HAUS Augmin-Like Complex, Subunit 1 (HAUS1) is a crucial component of the human augmin complex, also known as the HAUS complex. This complex plays a significant role in the assembly and maintenance of the mitotic spindle, which is essential for accurate chromosome segregation during cell division.

Discovery and Nomenclature

The augmin complex was first identified in the fruit fly, Drosophila melanogaster. The name “augmin” is derived from the Latin verb “augmentare,” meaning “to increase,” reflecting its role in enhancing microtubule generation within the mitotic spindle .

Structure and Composition

The HAUS complex is composed of eight subunits, including HAUS1. The other subunits are HAUS2, HAUS3, HAUS4, HAUS5, HAUS6, HAUS7, and HAUS8. HAUS1, also known as CCDC5 (Coiled-Coil Domain Containing 5), is a protein-coding gene located on chromosome 18q21.1 .

Function

The primary function of the HAUS complex is to facilitate the nucleation of microtubules within the mitotic spindle. This is achieved by recruiting the γ-tubulin ring complex (γ-TuRC) to pre-existing microtubules, thereby increasing microtubule density and ensuring the robustness of the spindle structure. HAUS1, as part of this complex, contributes to the maintenance of centrosome integrity and the completion of cytokinesis.

Biological Significance

The HAUS complex is vital for proper cell division. Disruption of any of the HAUS subunits, including HAUS1, can lead to severe mitotic defects, such as disorganized spindles, fragmentation of centrosomes, and increased centrosome size. These defects can result in chromosomal instability, which is a hallmark of many cancers.

Research and Clinical Implications

Research on the HAUS complex, including HAUS1, has provided valuable insights into the mechanisms of mitotic spindle assembly and the maintenance of genomic stability. Understanding the role of HAUS1 in cell division can have significant implications for cancer research, as targeting components of the mitotic machinery could offer new therapeutic strategies for cancer treatment.

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HAUS1 Human

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