HAPLN1 Human, HEK
HEK293 Cells.
Hyaluronan and proteoglycan link protein 1 precursor, CRTL1, Cartilage-linking protein 1, Cartilage-link protein, Proteoglycan link protein, HAPLN1.
Greater than 95.0% as determined by SDS-PAGE.
Description
HAPLN1 Human Recombinant produced in HEK293 Cells.is a single, glycosylated polypeptide chain containing 345 amino acids (16-354 a.a.) and having a molecular mass of 39.3kDa. HAPLN1 is fused to a 6 amino acid His-tag at C-terminus and is purified by proprietary chromatographic techniques.
Product Specs
Hyaluronan and proteoglycan link protein 1 precursor, CRTL1, Cartilage-linking protein 1, Cartilage-link protein, Proteoglycan link protein, HAPLN1.
HEK293 Cells.
DHLSDNYTLD HDRAIHIQAE NGPHLLVEAE QAKVFSHRGG NVTLPCKFYR DPTAFGSGIH KIRIKWTKLT SDYLKEVDVF VSMGYHKKTY GGYQGRVFLK GGSDSDASLV ITDLTLEDYG RYKCEVIEGL EDDTVVVALD LQGVVFPYFP RLGRYNLNFH EAQQACLDQD AVIASFDQLY DAWRGGLDWC NAGWLSDGSV QYPITKPREP CGGQNTVPGV RNYGFWDKDK SRYDVFCFTS NFNGRFYYLI HPTKLTYDEA VQACLNDGAQ IAKVGQIFAA WKILGYDRCD AGWLADGSVR YPISRPRRRC SPTEAAVRFV GFPDKKHKLY GVYCFRAYNH HHHHH.
Product Science Overview
Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1), also known as Cartilage Link Protein 1 (Crtl-1), is a crucial component of the extracellular matrix (ECM). This protein plays a significant role in maintaining the structural integrity of various tissues, particularly cartilage. HAPLN1 is a glycoprotein composed of 354 amino acids and is essential for normal cartilage development .
HAPLN1 is involved in the stabilization of proteoglycan aggregates in the ECM. It binds to hyaluronan and proteoglycans, facilitating the formation of large, stable complexes that are critical for the resilience and elasticity of cartilage. This protein is also implicated in various cellular processes, including cell adhesion, migration, and proliferation .
The recombinant form of HAPLN1, produced in Human Embryonic Kidney (HEK) cells, is utilized for research and therapeutic purposes. HEK cells are preferred for recombinant protein production due to their ability to perform post-translational modifications similar to those in human cells. The recombinant HAPLN1 is produced by transfecting HEK cells with a plasmid containing the HAPLN1 gene, followed by protein expression and purification.
HAPLN1 has been associated with several diseases and conditions. For instance, its loss has been linked to tumorigenesis in colorectal cancer. Studies have shown that the mRNA expression of HAPLN1 is decreased in tumors from colorectal cancer patients compared to healthy controls . Additionally, HAPLN1 is involved in the regulation of the Transforming Growth Factor (TGF)-β signaling pathway, which controls collagen deposition and tumor growth .
In the context of respiratory health, a specific single-nucleotide polymorphism (SNP) in the HAPLN1 gene has been associated with the need for home oxygen therapy in premature infants with bronchopulmonary dysplasia . This highlights the diverse roles of HAPLN1 in different physiological and pathological processes.
Recombinant HAPLN1 is widely used in research to study its role in ECM biology, cancer, and other diseases. It serves as a valuable tool for investigating the molecular mechanisms underlying its functions and for developing potential therapeutic strategies targeting HAPLN1-related pathways.
