HAO1 Mouse
(S)-2-hydroxy-acid oxidase; EC 1.1.3.15, Glycolate oxidase, GOX, GOX1MGC142227;
GOXMGC142225, HAO1, HAO-1, HAOX1, hydroxyacid oxidase (glycolate oxidase) 1, hydroxyacid oxidase 1, Hydroxyacid Oxidase1, Hydroxyacid Oxidase-1.
Greater than 90.0% as determined by SDS-PAGE.
Description
HAO1 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 393 amino acids (1-370) and having a molecular mass of 43.4 kDa.
HAO1 is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
(S)-2-hydroxy-acid oxidase; EC 1.1.3.15, Glycolate oxidase, GOX, GOX1MGC142227;
GOXMGC142225, HAO1, HAO-1, HAOX1, hydroxyacid oxidase (glycolate oxidase) 1, hydroxyacid oxidase 1, Hydroxyacid Oxidase1, Hydroxyacid Oxidase-1.
MGSSHHHHHH SSGLVPRGSH MGSMLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI.
Product Science Overview
Hydroxyacid Oxidase 1 (HAO1), also known as glycolate oxidase, is an enzyme that belongs to the superfamily of alpha hydroxy acid oxidases. This enzyme plays a crucial role in the oxidation of glycolate to glyoxylate and glyoxylate to oxalate, with the concomitant reduction of oxygen to hydrogen peroxide .
HAO1 is a single, non-glycosylated polypeptide chain consisting of 393 amino acids, with a molecular mass of approximately 43.4 kDa. The recombinant form of HAO1 is produced in Escherichia coli and is fused to a 23 amino acid His-Tag at the N-terminus . The enzyme is primarily expressed in the liver and pancreas, where it exhibits the highest activity on two-carbon substrates such as glycolate .
The primary function of HAO1 is to catalyze the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate. This reaction is significant as it helps in the metabolism of glycolate, a byproduct of various metabolic pathways. The enzyme’s activity is crucial in preventing the accumulation of glycolate and glyoxylate, which can lead to the formation of calcium oxalate crystals and kidney stones .
The recombinant form of HAO1 is produced using Escherichia coli as the host organism. The production process involves the expression of the HAO1 gene in E. coli, followed by purification using proprietary chromatographic techniques. The recombinant enzyme is available in various quantities and is used for laboratory research purposes .
The recombinant HAO1 protein is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol. It is recommended to store the enzyme at 4°C if it will be used within 2-4 weeks. For long-term storage, the enzyme should be frozen at -20°C, and it is advisable to add a carrier protein to prevent degradation .
