HAAO Human
3-Hydroxyanthranilate 3,4-Dioxygenase Human Recombinant
Cat. No.
BT19954
Source
Escherichia Coli.
Synonyms
3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-HAO, 3-hydroxyanthranilic acid dioxygenase, HAD, HAAO, HAO.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HAAO Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (1-286 a.a.) and having a molecular mass of 35kDa.
HAAO is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
HAAO is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
HAAO (3-hydroxyanthranilate 3,4-dioxygenase) is a monomeric cytosolic enzyme that belongs to the intramolecular dioxygenase family. These enzymes are characterized by their use of nonheme ferrous iron as a cofactor. HAAO specifically catalyzes the conversion of 3-hydroxyanthranilic acid into quinolinic acid (QUIN). QUIN is recognized as an excitotoxin due to its ability to activate glutamate N-methyl-D-aspartate receptors, leading to neuronal overexcitation. Elevated levels of QUIN in the brain have been implicated in the development of neurological and inflammatory disorders. While HAAO is found in high concentrations in peripheral organs like the liver and kidneys, it is also present in lower amounts within the central nervous system.
Description
Recombinantly produced in E. coli, HAAO Human is a single, non-glycosylated polypeptide chain. It consists of 310 amino acids, with the first 286 amino acids corresponding to the HAAO sequence. The protein has a molecular weight of 35 kDa. For purification and detection purposes, a 24 amino acid His-tag is fused to the N-terminus. The purification process involves proprietary chromatographic techniques.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
The HAAO protein is supplied in a solution with a concentration of 0.5 mg/ml. The solution contains the following components: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It's important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the HAAO protein is determined by SDS-PAGE analysis and is guaranteed to be greater than 90.0%.
Synonyms
3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-HAO, 3-hydroxyanthranilic acid dioxygenase, HAD, HAAO, HAO.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMERRLG VRAWVKENRG SFQPPVCNKL MHQEQLKVMF IGGPNTRKDY HIEEGEEVFY QLEGDMVLRV LEQGKHRDVV IRQGEIFLLP ARVPHSPQRF ANTVGLVVER RRLETELDGL RYYVGDTMDV LFEKWFYCKD LGTQLAPIIQ EFFSSEQYRT GKPIPDQLLK EPPFPLSTRS IMEPMSLDAW LDSHHRELQA GTPLSLFGDT YETQVIAYGQ GSSEGLRQNV DVWLWQLEGS SVVTMGGRRL SLAPDDSLLV LAGTSYAWER TQGSVALSVT QDPACKKPLG.
Product Science Overview
Introduction
3-Hydroxyanthranilate 3,4-Dioxygenase (3HAO), also known as HAAO, is a crucial enzyme in the kynurenine pathway of tryptophan degradation. This pathway is significant for the production of several bioactive metabolites, including quinolinic acid (QUIN), which has implications in various neurological and inflammatory disorders .
Classification
Biological Properties
Functions
The primary function of 3HAO is to catalyze the cleavage of the benzene ring of 3-hydroxyanthranilic acid (3-Ohaa), an intermediate in the kynurenine pathway . This reaction is essential for the synthesis of quinolinic acid, an excitotoxin that can activate glutamate N-methyl-D-aspartate (NMDA) receptors . Elevated levels of quinolinic acid are associated with several neurological and inflammatory disorders .
Modes of Action
Regulatory Mechanisms
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