GUK1 Human, Active
Guanylate Kinase 1 Human Recombinant, Active
Cat. No.
BT24505
Source
Escherichia Coli.
Synonyms
GMK, GMP kinase.
Appearance
Sterile Filtered colorless clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GUK1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (1-197 a.a.)and having a total molecular mass of 23.9 kDa.
GUK1 is fused to a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.
GUK1 is fused to a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.
Product Specs
Introduction
GUK1, a member of the guanylate kinase family, functions as a monomer, catalyzing the ATP-dependent conversion of GMP to GDP. This enzymatic activity plays a crucial role in GMP recycling and the regulation of guanine nucleotide supply for signal transduction pathways. Notably, GUK1 overexpression has been linked to pituitary adenocarcinomas, suggesting its potential involvement in tumor development.
Description
Recombinant human GUK1, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 217 amino acids (1-197 a.a.) with a molecular weight of 23.9 kDa. This protein features a 20 amino acid His Tag at the N-terminus and undergoes purification through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized through filtration.
Formulation
The GUK1 protein solution, provided at a concentration of 1 mg/ml, is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8), 1 mM DTT, 0.1 M NaCl, and 10% Glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C in a frozen state is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of this product is greater than 90%, as determined by SDS-PAGE analysis.
Biological Activity
Exhibiting a specific activity exceeding 100 units/mg, this enzyme is capable of converting 1.0 μmole of GMP and ATP to GDP and ADP per minute at pH 7.5 and 37°C when measured in a coupled system with PK/LDH.
Synonyms
GMK, GMP kinase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA.
Product Science Overview
Gene and Protein Information
- Gene Name: GUK1
- Protein Class: Enzymes, Metabolic proteins
- Gene Symbol: GUK1
- HGNC ID: 4693
- NCBI Gene ID: 2987
- UniProtKB/Swiss-Prot ID: Q16774
Biological Functions
GUK1 is involved in several critical cellular processes:
- Nucleotide Metabolism: It is essential for the recycling of GMP and indirectly, cyclic GMP (cGMP), which is vital for various cellular functions .
- Signal Transduction: By regulating the supply of guanine nucleotides, GUK1 plays a role in signal transduction pathways .
- Photoreceptor Function: GUK1 is involved in cGMP metabolism in photoreceptors, which is crucial for vision .
Clinical Significance
Expression and Localization
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