GSTP1 Antibody
Glutathione S-transferase P 1, Gst P1, GST YF-YF, GST class-pi, GST-piB, Preadipocyte growth factor.
Description
Product Specs
GSTP1, a polymorphic gene, encodes active GSTP1 variant proteins with functional differences. These proteins are believed to play a role in xenobiotic metabolism and may contribute to susceptibility to cancer and other diseases. As a glutathione S-transferase belonging to the pi class, GSTP1 is part of the GST family, whose enzymes are crucial for detoxification. They catalyze the conjugation of various hydrophobic and electrophilic compounds with reduced glutathione. Soluble GSTs are classified into four main classes based on their biochemical, immunological, and structural properties: alpha, mu, pi, and theta. GSTP1 catalyzes the reaction between glutathione and an acceptor molecule, forming Sulfur-substituted glutathione. Reactions involving glutathione contribute to transforming a wide range of electrophiles, such as reactive products of lipids, proteins, carcinogens, therapeutic drugs, environmental toxins, and oxidative stress products. Frequent inactivation of GSTP1 due to CpG hypermethylation in pituitary adenomas suggests a potential role in aggressive tumor behavior. Notably, GSTP1 may be a transcriptional target of the tumor suppressor gene p53. Single-nucleotide polymorphisms in GSTP1 are linked to altered protein binding, influencing its role in carcinogen and drug metabolism, potentially impacting disease pathogenesis and drug response. GST-pi might play a central role in the proliferation of androgen-independent prostate cancer cells.
The solution contains 1mg/ml of GSTP1 antibody in a buffer consisting of PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
The GSTP1 antibody has been validated for use in various applications, including ELISA, Western blot analysis, ICC/IF, and Flow cytometry. These tests confirm its specificity and reactivity. However, it is important to note that optimal antibody dilutions should be determined empirically for each specific application to achieve the best results.
Glutathione S-transferase P 1, Gst P1, GST YF-YF, GST class-pi, GST-piB, Preadipocyte growth factor.
GSTP1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT12C10AT
Anti-human GSTP1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GSTP1 amino acids 1-210 purified from E. coli.
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Glutathione S-Transferase pi 1 (GSTP1) is a member of the glutathione S-transferase (GST) family, which plays a crucial role in the detoxification of endogenous and exogenous compounds. GSTP1 is involved in the conjugation of glutathione to various electrophilic compounds, including metabolites generated by oxidative processes, environmental toxins, carcinogens, and anti-cancer drugs .
GSTP1 is highly polymorphic and encodes functionally different variant proteins that are thought to be involved in xenobiotic metabolism and susceptibility to cancer and other diseases . In humans, GSTP1 expression is found in various tissues, including the liver, where it is primarily expressed in non-parenchymal cells such as bile duct cells, Kupffer cells, macrophages, and endothelial cells . In contrast, mouse GSTP1 (mGstp) is highly expressed in hepatocytes .
The Mouse Anti-Human GSTP1 antibody is a monoclonal antibody that specifically detects human GSTP1 in various applications, including Western blotting, immunocytochemistry, and ELISA . This antibody is produced by immunizing mice with recombinant human GSTP1 protein and is purified using protein A or G from hybridoma culture supernatant .
The Mouse Anti-Human GSTP1 antibody is widely used in research to study the expression and function of GSTP1 in different tissues and under various conditions. For example, it has been used to detect GSTP1 in human cervical epithelial carcinoma cell lines (HeLa) and human embryonic stem cells differentiated into hepatocytes . Additionally, this antibody has been employed in studies investigating the role of GSTP1 in liver toxicity and drug metabolism .
