GSS Antibody
Description
Product Specs
Product Science Overview
Glutathione synthetase (GSS) is an essential enzyme in the biosynthesis of glutathione, a critical antioxidant that protects cells from oxidative stress and maintains redox balance. The enzyme catalyzes the ATP-dependent conversion of γ-glutamylcysteine and glycine to glutathione. Glutathione synthetase is encoded by the GSS gene and is highly conserved across species, including humans and mice.
Mouse anti-human glutathione synthetase antibodies are monoclonal antibodies produced by immunizing mice with human GSS protein. These antibodies are designed to specifically bind to human GSS, allowing researchers to detect and quantify the enzyme in various biological samples. The antibodies are commonly used in immunohistochemistry (IHC), Western blotting (WB), and enzyme-linked immunosorbent assays (ELISA).
The preparation of mouse anti-human glutathione synthetase antibodies involves several key steps:
- Immunization: Mice are immunized with recombinant human GSS protein to elicit an immune response.
- Hybridoma Production: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells that produce monoclonal antibodies.
- Screening and Selection: Hybridoma cells are screened for the production of antibodies that specifically bind to human GSS. Positive clones are selected and expanded.
- Purification: The monoclonal antibodies are purified from the hybridoma cell culture supernatant using protein A or protein G affinity chromatography.
Mouse anti-human glutathione synthetase antibodies have several important applications in biomedical research:
- Immunohistochemistry (IHC): These antibodies are used to detect GSS expression in tissue sections, providing insights into the enzyme’s distribution and localization in different tissues.
- Western Blotting (WB): The antibodies are employed to detect and quantify GSS protein levels in cell lysates and tissue extracts, aiding in the study of protein expression and regulation.
- ELISA: These antibodies are utilized in ELISA assays to measure GSS concentrations in biological samples, facilitating research on glutathione metabolism and related diseases.
