Introduction
Glutathione reductase (GSR) is an enzyme that helps maintain the balance of glutathione (GSH), an important antioxidant in cells. It belongs to the pyridine nucleotide-disulfide oxidoreductase family and functions as a homodimer with a flavin molecule. GSR catalyzes the reduction of glutathione disulfide (GSSG) back to its reduced form (GSH) using NADPH as a cofactor. This reaction is crucial for detoxifying reactive oxygen species and supporting various cellular processes, including protein and DNA synthesis.
Physical Appearance
A clear and colorless liquid that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a buffer solution containing phosphate-buffered saline (PBS) at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
The product can be stored at 4°C for up to one month. For longer-term storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles to maintain product integrity.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This GSR antibody has undergone testing through ELISA and Western blot analysis to confirm its specificity and reactivity. As specific experimental conditions may vary, it is advisable to determine the optimal working concentration for each application. A starting dilution of 1:500 is recommended for initial experiments.
Synonyms
Glutathione reductase mitochondrial, GR, GRase, GSR, GLUR, GRD1.
Purification Method
GSR antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human GSR mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GSR amino acids 43-522 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
