GroEL Antibody

GroEL, also known as Heat Shock Protein 60 (HSP60), is a molecular chaperonin that plays a crucial role in protein folding and maintaining cellular homeostasis. It is highly conserved across different species, from prokaryotes to eukaryotes. In prokaryotes, GroEL functions as part of the GroEL/GroES complex, which forms a double-ring structure that assists in the proper folding of proteins .

GroEL is a 60 kDa protein that forms a complex with GroES, another chaperonin, to create a functional unit essential for protein folding. This complex operates by encapsulating unfolded or misfolded proteins within its central cavity, providing an isolated environment where proteins can achieve their correct conformation. This process is ATP-dependent and involves multiple cycles of binding and release .

In eukaryotes, HSP60 is located in the mitochondria and is involved in the folding of mitochondrial proteins. It also plays a role in protecting cells from stress by preventing the aggregation of misfolded proteins. HSP60 is implicated in various cellular processes, including apoptosis, oxidative stress response, and immune response .

HSP60 has been studied extensively for its role in various diseases. It is considered a potential therapeutic target for conditions such as cancer, neurodegenerative diseases, and inflammatory disorders. The protein’s involvement in maintaining mitochondrial integrity and its role in apoptosis make it a critical factor in cell survival and disease progression .

The mouse anti-human GroEL (HSP60) antibody is a monoclonal antibody that specifically targets the human HSP60 protein. This antibody is used in various research applications, including Western blotting, immunohistochemistry, and immunofluorescence. It is particularly useful in distinguishing human HSP60 from its bacterial and other mammalian homologs .

The antibody is produced by immunizing mice with recombinant human HSP60 protein, leading to the generation of a specific immune response. The resulting monoclonal antibody is then purified and characterized for its specificity and affinity towards human HSP60 .

The mouse anti-human GroEL (HSP60) antibody is widely used in research to study the expression and localization of HSP60 in different tissues and under various conditions. It helps in understanding the role of HSP60 in disease mechanisms and can be used to explore potential therapeutic interventions. For example, in cancer research, this antibody can be used to investigate the expression levels of HSP60 in tumor tissues, providing insights into the protein’s role in tumorigenesis and potential as a therapeutic target .