GP2 Human
Greater than 95% as determined by SDS-PAGE.
Description
GP2 is a cDNA coding for the free form of the human pancreatic secretory granule membrane major glycoprotein / GP2 having a molecular mass of 68,000 Dalton (protein component excluding glycosylation; observed molecular weight approx. 68 kDa) pH 5.4. GP2 protein is fused to a deca-histidine purification tag.
Product Specs
Crohn's Disease (CD) is an inflammatory bowel disease (IBD) prevalent among Caucasians. It is characterized by mucosal inflammation, believed to result from an immune system imbalance. This imbalance disrupts the harmony between tolerance towards commensal microbiota and food antigens and the immune response against pathogens. Autoimmune processes are implicated in CD development, with exocrine pancreas autoantibodies (PABs) serving as specific markers. Glycoprotein 2 (GP2) has been recently identified as the primary autoantigenic target recognized by CD-specific PABs. Alongside IgG and IgM PAB isotypes, IgA pancreatic autoantibodies have also been detected in individuals with CD. GP2 is a heavily glycosylated protein, with a molecular weight of 78 kDa, characterized by N-linked carbohydrates. It constitutes up to 40% of the total zymogen granule (ZG) membrane proteins within pancreatic acinar cells and anchors to the ZG membrane through a glycosylphosphatidylinositol (GPI) anchor.
This product consists of cDNA encoding the human pancreatic secretory granule membrane major glycoprotein, GP2, in its free form. The protein has a molecular mass of 68 kDa (excluding glycosylation) and an observed molecular weight of approximately 68 kDa at pH 5.4. A deca-histidine purification tag is fused to the GP2 protein.
The GP2 solution is provided in a buffer consisting of 16mM HEPES (pH 7.5), 80mM NaCl, and 20% glycerol.
For optimal preservation, store the product at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze at -20°C. Repeated freezing and thawing cycles should be avoided.
Purity is determined to be greater than 95% based on SDS-PAGE analysis.
Product Science Overview
Glycoprotein-2 (GP2) is a significant protein found in various tissues, particularly in the digestive tract. It plays a crucial role in maintaining mucosal barrier integrity and immune homeostasis. The recombinant form of this protein, known as Human Recombinant Glycoprotein-2, is produced using advanced biotechnological methods to study its structure and function in detail.
GP2 is a glycosylated protein, meaning it has carbohydrate groups attached to its polypeptide chain. This glycosylation is essential for its proper folding, stability, and function. In humans, GP2 is primarily expressed in the pancreas and the gut, where it contributes to the mucosal barrier and immune responses .
The production of recombinant glycoproteins, including GP2, involves using eukaryotic expression systems to ensure proper glycosylation. This process typically involves the following steps :
- Gene Cloning: The gene encoding GP2 is cloned into an expression vector.
- Transfection: The vector is introduced into a suitable host cell line, such as mammalian cells.
- Protein Expression: The host cells produce the recombinant GP2, which undergoes glycosylation in the endoplasmic reticulum and Golgi apparatus.
- Purification: The recombinant protein is purified using techniques like affinity chromatography to obtain a high-purity product.
Recombinant GP2 is used in various research applications, including:
- Structural Studies: Understanding the three-dimensional structure of GP2 and its glycosylation patterns.
- Functional Analysis: Investigating the role of GP2 in mucosal immunity and its interactions with other proteins.
- Disease Research: Studying the involvement of GP2 in diseases like inflammatory bowel disease (IBD) and its potential as a therapeutic target .
