GOPC Human

Golgi-Associated PDZ and Coiled-Coil Motif Containing Human Recombinant
Cat. No.
BT13163
Source
Escherichia Coli.
Synonyms
CAL, dJ94G16.2, FIG, GOPC1, PIST, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma, GOPC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GOPC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (278-454 a.a.) and having a molecular mass of 21.5 kDa. The GOPC is fused to a 20 amino acid His Tag and purified by conventional chromatography.

Product Specs

Introduction
GOPC, a Golgi protein containing a PDZ domain, exhibits sequence-specific binding to the extreme C-terminus of proteins. It plays a role in intracellular protein trafficking and degradation. GOPC regulates both CFTR chloride currents and acid-induced ACCN3 currents by modulating the cell surface expression of these channels. Furthermore, it regulates the intracellular trafficking of the ADR1B receptor and participates in autophagy. Overexpression of GOPC leads to intracellular retention and lysosomal degradation of CFTR.
Description
Recombinant human GOPC, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 198 amino acids (278-454 a.a.). With a molecular mass of 21.5 kDa, it encompasses amino acids 278-454 of the GOPC protein and incorporates a 20-amino acid His tag at its N-terminus. Purification is achieved through conventional chromatography techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The GOPC protein solution is supplied in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), maintain the product at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 90.0% via SDS-PAGE analysis.
Synonyms
CAL, dJ94G16.2, FIG, GOPC1, PIST, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma, GOPC.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MIRKVLLLKE DHEGLGISIT GGKEHGVPIL ISEIHPGQPA DRCGGLHVGD AILAVNGVNL RDTKHKEAVT ILSQQRGEIE FEVVYVAPEV DSDDENVEYE DESGHRYRLY LDELEGGGNP GASCKDTSGE IKVLQGFNKK AVTDTHENGD LGTASETPLD DGASKLDDLH TLYHKKSY.

Product Science Overview

Structure and Domains

GOPC contains two main structural domains:

  1. PDZ Domain: This domain is approximately 90 amino acids long and is known for binding the extreme C-terminus of target proteins in a sequence-specific manner . PDZ domains are globular and facilitate protein-protein interactions.
  2. Coiled-Coil Motif: This motif is involved in the oligomerization of proteins, allowing GOPC to form homodimers or interact with other proteins .
Function and Mechanism

GOPC plays a crucial role in intracellular protein trafficking and degradation . It is involved in the regulation of several key proteins and receptors:

  • CFTR (Cystic Fibrosis Transmembrane Conductance Regulator): GOPC regulates CFTR chloride currents and its cell surface expression . Overexpression of GOPC can lead to CFTR intracellular retention and degradation in lysosomes .
  • ASIC3 (Acid-Sensing Ion Channel 3): GOPC modulates acid-induced ASIC3 currents by affecting its cell surface expression .
  • ADR1B (Adrenergic Receptor Beta 1): GOPC is involved in the intracellular trafficking of this receptor .

Additionally, GOPC has been implicated in autophagy, a cellular process that degrades and recycles cellular components . It mediates the ubiquitination and lysosomal degradation of CFTR in conjunction with MARCHF2 .

Clinical Significance

Mutations or dysregulation of the GOPC gene have been associated with several diseases:

  • Spermatogenic Failure 6: This condition is characterized by male infertility due to globozoospermia, a defect in sperm morphology .
  • Male Infertility Due to Globozoospermia: Similar to spermatogenic failure, this condition results from abnormal sperm development .
Research and Applications

Research on GOPC has provided insights into its role in various cellular processes and its potential as a therapeutic target. The recombinant form of GOPC is used in research to study its interactions with other proteins and its involvement in diseases.

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