GOLM1 Human

Golgi Membrane Protein 1 Human Recombinant
Cat. No.
BT13074
Source
Escherichia Coli.
Synonyms
Golgi Membrane Protein 1, Chromosome 9 Open Reading Frame 155, Epididymis Luminal Protein 46, Golgi Membrane Protein GP73, Golgi Phosphoprotein 2, GOLPH2, Golgi Protein 73-KD, PSEC0257, bA379P1.3, HEL46, C9orf155, GP73.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GOLM1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 389 amino acids (36-401 a.a) and having a molecular mass of 44.0kDa.
GOLM1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GOLM1, a type II Golgi transmembrane protein, plays a crucial role in protein processing and transport. It processes proteins synthesized in the rough endoplasmic reticulum and facilitates their movement through the Golgi apparatus. Upregulated during viral infections, GOLM1 serves as a potential molecular biomarker for cancer due to its tightly regulated cellular expression.
Description
Recombinant human GOLM1, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 389 amino acids (residues 36-401) with a molecular weight of 44.0 kDa. This protein is engineered with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile solution after filtration.
Formulation
The GOLM1 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the protein can be stored at 4°C. For extended storage, it is recommended to store frozen at -20°C. To ensure long-term stability, the addition of a carrier protein like HSA or BSA (0.1%) is recommended. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the GOLM1 protein is determined to be greater than 85% through SDS-PAGE analysis.
Synonyms
Golgi Membrane Protein 1, Chromosome 9 Open Reading Frame 155, Epididymis Luminal Protein 46, Golgi Membrane Protein GP73, Golgi Phosphoprotein 2, GOLPH2, Golgi Protein 73-KD, PSEC0257, bA379P1.3, HEL46, C9orf155, GP73.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSSRSVDL QTRIMELEGR VRRAAAERGA VELKKNEFQG ELEKQREQLD KIQSSHNFQL ESVNKLYQDE KAVLVNNITT GERLIRVLQD QLKTLQRNYG RLQQDVLQFQ KNQTNLERKF SYDLSQCINQ MKEVKEQCEE RIEEVTKKGN EAVASRDLSE NNDQRQQLQA LSEPQPRLQA AGLPHTEVPQ GKGNVLGNSK SQTPAPSSEV VLDSKRQVEK EETNEIQVVN EEPQRDRLPQ EPGREQVVED RPVGGRGFGG AGELGQTPQV QAALSVSQEN PEMEGPERDQ LVIPDGQEEE QEAAGEGRNQ QKLRGEDDYN MDENEAESET DKQAALAGND RNIDVFNVED QKRDTINLLD QREKRNHTL

Product Science Overview

Gene and Protein Structure

The GOLM1 gene is located on chromosome 9 (9q21.33) in humans . It encodes a protein that is approximately 400 amino acids in length and has a molecular weight of around 73 kDa . The protein consists of a single transmembrane domain, with the N-terminus located in the cytoplasm and the C-terminus in the lumen of the Golgi apparatus .

Function

GOLM1 is involved in the processing and transport of proteins synthesized in the rough endoplasmic reticulum . It is particularly important in the post-translational modification of proteins, including glycosylation, which is essential for the proper functioning of many proteins . The expression of GOLM1 is upregulated in response to viral infections, suggesting a role in the cellular response to viral pathogens .

Clinical Significance

GOLM1 has been implicated in various diseases, particularly those affecting the liver. It is overexpressed in liver diseases such as autoimmune hepatitis and liver cancer . Elevated levels of GOLM1 have been observed in the blood of patients with liver cancer, making it a potential biomarker for the early detection of this disease . Additionally, GOLM1 is overexpressed in prostate cancer and lung adenocarcinoma .

Recombinant GOLM1

Recombinant GOLM1 is produced using recombinant DNA technology, where the GOLM1 gene is cloned into an expression vector and introduced into a host cell, typically E. coli or mammalian cells . The host cells then produce the GOLM1 protein, which can be purified and used for various research and diagnostic purposes . Recombinant GOLM1 is valuable for studying the protein’s function, interactions, and role in disease, as well as for developing diagnostic assays and potential therapeutic interventions .

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