GNMT Human, Active
Escherichia Coli.
Glycine N-methyltransferase, GNMT, Glycine N-Methyltransferase, EC 2.1.1.20
Epididymis Secretory Sperm Binding Protein Li 182mP, HEL-S-182mP.
Greater than 95.0% as determined by SDS-PAGE.
Description
GNMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295 a.a) and having a molecular mass of 34.9kDa.
GNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Glycine N-methyltransferase, GNMT, Glycine N-Methyltransferase, EC 2.1.1.20
Epididymis Secretory Sperm Binding Protein Li 182mP, HEL-S-182mP.
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD.
Product Science Overview
Glycine N-Methyltransferase (GNMT) is an enzyme that plays a crucial role in the metabolism of amino acids. It is involved in the methylation process, specifically catalyzing the conversion of glycine to sarcosine using S-adenosyl-L-methionine (AdoMet) as a methyl donor . This reaction also produces S-adenosyl-L-homocysteine (AdoHcy) as a byproduct .
GNMT is a cytoplasmic enzyme that functions as a homotetramer . Each subunit of the tetramer contributes to the enzyme’s overall activity. The enzyme’s primary function is to regulate the levels of AdoMet and AdoHcy, which are critical for various methylation reactions in the body . By controlling the ratio of these compounds, GNMT helps maintain the balance of methyl groups within cells .
The GNMT gene is located on chromosome 6 in humans . Mutations or defects in this gene can lead to GNMT deficiency, a condition characterized by elevated levels of methionine in the blood (hypermethioninemia) . This deficiency can have various metabolic consequences and may require medical intervention.
GNMT is involved in several metabolic pathways, including one-carbon metabolism and methionine metabolism . One-carbon metabolism is essential for the synthesis of nucleotides and the regulation of gene expression. Methionine metabolism, on the other hand, is crucial for the production of vital compounds such as cysteine, taurine, and glutathione .
Due to its role in regulating methylation reactions, GNMT has been studied for its potential implications in various diseases. For instance, alterations in GNMT activity have been linked to liver diseases, cancer, and neurological disorders . Understanding the enzyme’s function and regulation can provide insights into the development of therapeutic strategies for these conditions.
Recombinant human GNMT is produced using genetic engineering techniques to express the enzyme in host cells, such as bacteria or yeast . This recombinant form is used in research to study the enzyme’s properties, functions, and potential applications in medicine. The active form of recombinant GNMT retains the same catalytic activity as the naturally occurring enzyme, making it a valuable tool for scientific investigations .
