GNG4 Human

GNG4 is a subunit of the G protein complex, which consists of alpha, beta, and gamma subunits. The gamma subunit, along with the beta subunit, is essential for the GTPase activity, the replacement of GDP by GTP, and the interaction with G protein effectors . The GNG4 gene is involved in defining the specificity of receptor-G protein interaction .

GNG4 is involved in several important pathways, including:

• ADORA2B mediated anti-inflammatory cytokines production
• Thromboxane signaling through TP receptor

These pathways highlight the role of GNG4 in inflammatory responses and other critical cellular processes.

The human recombinant form of GNG4 is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 95 amino acids. It has a molecular mass of approximately 10.4 kDa and is fused to a 23 amino acid His-tag at the N-terminus . The recombinant protein is typically used in research to study its function and interactions in various biological processes.

The recombinant GNG4 protein is stored at -20°C for long-term stability. It is recommended to add a carrier protein, such as 0.1% HSA or BSA, to prevent multiple freeze-thaw cycles .

GNG4’s role in signal transduction makes it a significant target for research in various fields, including cancer, cardiovascular diseases, and neurological disorders. Understanding the function and regulation of GNG4 can provide insights into the development of therapeutic strategies for these conditions.