GNG13 Human

Guanine Nucleotide Binding Protein Gamma 13 Human Recombinant
Cat. No.
BT13307
Source
Escherichia Coli.
Synonyms
Guanine Nucleotide Binding Protein (G Protein), Gamma 13, Clone:H2-35, G Gamma Subunit, G(gamma)13, Guanine Nucleotide Binding Protein 13, Gamma, Guanine Nucleotide-Binding Protein G(I)/G(S)/G(O) Subunit Gamma-13 h2-35, G Gamma Subunit, clone:h2-35, GNG13.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GNG13 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 87 amino acids (1-64 a.a) and having a molecular mass of 10.0 kDa. 
GNG13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Guanine Nucleotide Binding Protein Gamma 13, also known as GNG13, is a member of the G protein gamma family. These proteins are part of a larger complex called heterotrimeric G proteins, which include alpha, beta, and gamma subunits. These G proteins act as signal messengers for G protein-coupled receptors, which are found on cell surfaces. GNG13, the gamma subunit, is primarily found in taste, retinal, and neuronal tissues, playing a crucial role in how we perceive taste.
Description
Recombinant human GNG13, produced in E. coli, is a single, non-glycosylated polypeptide chain. It contains 87 amino acids (specifically, amino acids 1-64), resulting in a molecular weight of 10.0 kDa. This GNG13 protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The GNG13 protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 40% glycerol, 2mM DTT, and 0.1mM PMSF.
Stability
For short-term storage (up to 2-4 weeks), the GNG13 protein should be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein (either 0.1% HSA or BSA) is advisable. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the GNG13 protein is greater than 80.0%, as determined by SDS-PAGE analysis.
Synonyms
Guanine Nucleotide Binding Protein (G Protein), Gamma 13, Clone:H2-35, G Gamma Subunit, G(gamma)13, Guanine Nucleotide Binding Protein 13, Gamma, Guanine Nucleotide-Binding Protein G(I)/G(S)/G(O) Subunit Gamma-13 h2-35, G Gamma Subunit, clone:h2-35, GNG13.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEEWDVP QMKKEVESLK YQLAFQREMA SKTIPELLKW IEDGIPKDPF LNPDLMKNNP WVEKGKC.

Product Science Overview

Introduction

Guanine Nucleotide Binding Protein Gamma 13 (GNG13) is a subunit of heterotrimeric G proteins, which are involved in various transmembrane signaling systems. These proteins play a crucial role as modulators or transducers in signal transduction pathways, particularly those linked to G protein-coupled receptors (GPCRs).

Structure and Function

GNG13 is part of the gamma subunit family of G proteins. Heterotrimeric G proteins consist of three subunits: alpha, beta, and gamma. The gamma subunit, along with the beta subunit, is essential for the GTPase activity, the replacement of GDP by GTP, and the interaction with G protein effectors .

The GNG13 protein is composed of 67 amino acids and has a molecular mass of approximately 7.9 kDa . It shares about 96% sequence identity with its mouse counterpart, indicating a high degree of conservation across species .

Expression and Localization

GNG13 is expressed in various tissues, with notable expression in the brain, retina, and taste receptor cells . In humans, it is strongly expressed in the brain and weakly in the small intestine and thymus . In mice, it is detected in the brain, retina, olfactory epithelium, stomach, and testis .

Role in Signal Transduction

GNG13, as part of the heterotrimeric G protein complex, is involved in the signal transduction pathways mediated by GPCRs. These pathways are critical for various physiological processes, including taste transduction . The gamma subunit, in conjunction with the beta subunit, facilitates the activation of the G protein by promoting the exchange of GDP for GTP on the alpha subunit .

Recombinant GNG13

Recombinant GNG13 protein is produced using various expression systems, including bacterial, yeast, baculovirus-insect, and mammalian systems . These recombinant proteins are used in research to study the function and interaction of GNG13 in signal transduction pathways. The recombinant human GNG13 protein typically includes a His-tag for purification purposes and is available in high purity for experimental use .

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