GNB3 Human
Guanine Nucleotide Binding Protein Beta Polypeptide 3 Human Recombinant
Cat. No.
BT13075
Source
Escherichia Coli.
Synonyms
Transducin Beta Chain 3, G Protein, Beta-3 Subunit, GTP-Binding
Regulatory Protein Beta-3 Chain, Guanine Nucleotide-Binding Protein
G(I)/G(S)/G(T) Beta Subunit 3, Guanine Nucleotide-Binding Protein
G(I)/G(S)/G(T) Subunit Beta-3, Hypertension Associated Protein, Guanine
Nucleotide Binding Protein (G Protein), Beta Polypeptide 3, GNB3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
GNB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 363 amino acids (1-340 a.a) and having a molecular mass of 39.6kDa.
GNB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
The GNB3 gene encodes a protein that is part of the WD repeat G protein beta family. This protein plays a crucial role in regulating the alpha subunit of G proteins, as well as in various signal transduction pathways. A specific single-nucleotide polymorphism (SNP), C825T, in the GNB3 gene has been associated with conditions such as hypertension and obesity. This SNP is also linked to the production of a splice variant known as GNB3-s, which exhibits increased activity. GNB3-s exemplifies how nucleotide changes outside of typical splice sites can influence alternative splicing. While other splice variants of GNB3 may exist, they haven't been fully characterized yet. GNB3 has been implicated in medical conditions including syncope and aortic coarctation.
Description
Recombinant human GNB3, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 363 amino acids (specifically, amino acids 1-340) and has a molecular weight of 39.6 kDa. The GNB3 protein includes a 23 amino acid His-tag fused to its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, sterile-filtered solution.
Formulation
The GNB3 protein solution is provided at a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and contains 10% glycerol and 0.4M Urea.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the GNB3 protein is determined to be greater than 80.0% using SDS-PAGE analysis.
Synonyms
Transducin Beta Chain 3, G Protein, Beta-3 Subunit, GTP-Binding
Regulatory Protein Beta-3 Chain, Guanine Nucleotide-Binding Protein
G(I)/G(S)/G(T) Beta Subunit 3, Guanine Nucleotide-Binding Protein
G(I)/G(S)/G(T) Subunit Beta-3, Hypertension Associated Protein, Guanine
Nucleotide Binding Protein (G Protein), Beta Polypeptide 3, GNB3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AKLWDVREGT CRQTFTGHES DINAICFFPN GEAICTGSDD ASCRLFDLRA DQELICFSHE SIICGITSVA FSLSGRLLFA GYDDFNCNVW DSMKSERVGI LSGHDNRVSC LGVTADGMAV ATGSWDSFLK IWN.
Product Science Overview
Introduction
Structure and Function
GNB3 belongs to the WD repeat G protein beta family. The protein consists of 340 amino acids and has a molecular mass of approximately 39.6 kDa . It is a non-glycosylated polypeptide chain produced in E. coli for recombinant purposes . The beta subunits, including GNB3, are essential regulators of the alpha subunits and certain signal transduction receptors and effectors .
Genetic Variants and Clinical Significance
A notable single-nucleotide polymorphism (SNP) in the GNB3 gene, known as C825T, is associated with essential hypertension and obesity . This SNP leads to the occurrence of a splice variant, GNB3-s, which exhibits increased activity. The alternative splicing caused by this nucleotide change results in multiple transcript variants .
Biological Pathways
GNB3 is involved in several critical biological pathways, including:
- G protein-coupled receptor (GPCR) signaling: GNB3 plays a pivotal role in transmitting signals from GPCRs to intracellular effectors .
- Regulation of blood pressure: The protein is implicated in the regulation of blood pressure, which is linked to its association with hypertension .
- Metabolic processes: GNB3 is involved in the regulation of glucose and lipid metabolism, influencing obesity and related metabolic disorders .
Expression and Localization
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
