GNB2L1 Human
Guanine Nucleotide Binding Protein beta polypeptide 2-like 1 Human Recombinant
Cat. No.
BT13007
Source
E.coli.
Synonyms
Guanine nucleotide binding protein (G protein) beta polypeptide 2-like 1, RACK1, Receptor for Activated C Kinase 1, Gnb2-rs1, H12.3, Cell proliferation-inducing gene 21 protein, Guanine nucleotide-binding protein subunit beta-like protein 12.3, Human lung cancer oncogene 7 protein, Receptor of activated protein kinase C 1, HLC-7, PIG21, proliferation-inducing gene 21, protein homologous to chicken B complex protein guanine nucleotide binding.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GNB2L1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-317 and having a molecular mass of 37.2kDa.
GNB2L1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
GNB2L1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Initially recognized as an anchoring protein for protein kinase C beta (PKCbeta), which it stabilizes in its active state and tethers to membranes or functional locations, GNB2L1 is now understood to play a crucial role in various vital biological processes. GNB2L1 exhibits specific binding to the activated form of PKCbeta isoforms and interacts with numerous other essential signaling proteins, including the androgen receptor, Src kinase family members, integrins beta1, beta2, beta3, and beta5, beta-spectrin, dynamin, RasGAP, IGF-1r, Epstein-Barr virus trans-activator protein BZLF1, p73, and pRB. GNB2L1 is abundantly expressed in tissues of higher mammals, such as the spleen, liver, and brain.
Description
Recombinant human GNB2L1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 337 amino acids (residues 1-317) with a molecular weight of 37.2 kDa. The protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The GNB2L1 solution is provided at a concentration of 0.25 mg/ml in a buffer composed of 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 5 mM DTT, and 50% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing cycles.
Purity
The purity of the protein is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Guanine nucleotide binding protein (G protein) beta polypeptide 2-like 1, RACK1, Receptor for Activated C Kinase 1, Gnb2-rs1, H12.3, Cell proliferation-inducing gene 21 protein, Guanine nucleotide-binding protein subunit beta-like protein 12.3, Human lung cancer oncogene 7 protein, Receptor of activated protein kinase C 1, HLC-7, PIG21, proliferation-inducing gene 21, protein homologous to chicken B complex protein guanine nucleotide binding.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA GYTDNLVRVW QVTIGTR
Product Science Overview
Structure and Function
The GNB1L protein contains six WD repeats, which are essential for the formation of heterotrimeric or multiprotein complexes . These complexes play a crucial role in various cellular processes, including:
- Cell Cycle Progression: GNB1L is involved in the regulation of the cell cycle, ensuring proper cell division and growth.
- Signal Transduction: It participates in intracellular signaling pathways, transmitting signals from cell surface receptors to intracellular targets.
- Apoptosis: GNB1L is implicated in programmed cell death, a vital process for maintaining cellular homeostasis.
- Gene Regulation: It also plays a role in the regulation of gene expression, influencing the transcription of specific genes .
Expression and Localization
GNB1L is highly expressed in the heart and is mapped to the region on chromosome 22q11 . This region is significant because it is deleted in DiGeorge syndrome, trisomic in derivative 22 syndrome, and tetrasomic in cat-eye syndrome. Therefore, GNB1L may contribute to the etiology of these disorders .
Clinical Significance
Mutations or deletions in the GNB1L gene are associated with several disorders, including:
Research and Applications
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