GNAI1 Human

Guanine Nucleotide Binding Protein Alpha Inhibiting Activity 1 Human Recombinant

Cat. No.

BT12770

Source

Escherichia Coli.

Synonyms

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1, Gi.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

GNAI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 377 amino acids (1-354 a.a.) and having a molecular mass of 42.7kDa.
GNAI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Guanine nucleotide-binding proteins (G proteins) are essential components of signal transduction pathways. These heterotrimeric proteins consist of alpha, beta, and gamma subunits. Among them, GNAI1 represents the alpha subunit of an inhibitory G protein complex. This protein plays a crucial role in transducing signals received at cell surface receptors to intracellular effectors. Its functions include guanine nucleotide binding, GTP hydrolysis, and interaction with other proteins. Notably, GNAI1 participates in a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase, an enzyme involved in cyclic AMP production. Moreover, GNAI1 contributes to the opening of atrial potassium channels.

Description

This product consists of the recombinant human GNAI1 protein produced in E.Coli. It is a single, non-glycosylated polypeptide chain containing 377 amino acids (specifically, amino acids 1 to 354). The protein has a molecular weight of 42.7 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.

Physical Appearance

The product appears as a sterile, colorless solution after filtration.

Formulation

The GNAI1 protein is supplied in a solution at a concentration of 1 mg/ml. The solution contains 20 mM Tris-HCl buffer at pH 8.0 and 10% glycerol.

Stability

For short-term storage (up to 2-4 weeks), keep the product refrigerated at 4°C. For long-term storage, freeze the product at -20°C. To ensure optimal stability during extended storage, consider adding a carrier protein like HSA or BSA (0.1% concentration). Repeated freezing and thawing cycles should be avoided.

Purity

SDS-PAGE analysis indicates that the purity of this product is greater than 90%.

Synonyms

Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, GNAI1, Gi.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGCTLSA EDKAAVERSK MIDRNLREDG EKAAREVKLL LLGAGESGKS TIVKQMKIIH EAGYSEEECK QYKAVVYSNT IQSIIAIIRA MGRLKIDFGD SARADDARQL FVLAGAAEEG FMTAELAGVI KRLWKDSGVQ ACFNRSREYQ LNDSAAYYLN DLDRIAQPNY IPTQQDVLRT RVKTTGIVET HFTFKDLHFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTDTSIILF LNKKDLFEEK IKKSPLTICY PEYAGSNTYE EAAAYIQCQF EDLNKRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKDCGLF.

Product Science Overview

Introduction

Guanine Nucleotide Binding Protein Alpha Inhibiting Activity 1 (GNAI1) is a protein encoded by the GNAI1 gene in humans. This protein is a part of the G protein family, which plays a crucial role in signal transduction by interacting with G protein-coupled receptors (GPCRs). The GNAI1 protein is specifically involved in inhibitory signaling pathways, where it functions to inhibit adenylate cyclase activity, thereby reducing the levels of cyclic AMP (cAMP) within the cell .

Structure and Function

GNAI1 is a heterotrimeric G protein composed of three subunits: alpha, beta, and gamma. The alpha subunit, which is encoded by the GNAI1 gene, binds guanine nucleotides (GTP and GDP) and possesses intrinsic GTPase activity. This subunit alternates between an active GTP-bound state and an inactive GDP-bound state. Upon activation by a GPCR, the alpha subunit releases GDP and binds GTP, leading to the dissociation of the G protein into its alpha and beta-gamma subunits. The alpha subunit then interacts with downstream effectors to propagate the signal .

Biological Role

The primary function of GNAI1 is to inhibit adenylate cyclase, an enzyme responsible for converting ATP to cAMP. By inhibiting adenylate cyclase, GNAI1 reduces the production of cAMP, a secondary messenger involved in various cellular processes such as metabolism, gene transcription, and cell growth. This inhibitory action is crucial for maintaining cellular homeostasis and regulating physiological responses to external stimuli .

Genetic and Clinical Significance

Mutations or dysregulation of the GNAI1 gene can lead to various disorders. For instance, alterations in GNAI1 have been associated with neurodevelopmental disorders characterized by hypotonia, impaired speech, and behavioral abnormalities. Additionally, GNAI1 has been implicated in certain cancers and other diseases where aberrant signaling pathways play a role.

Recombinant GNAI1

Recombinant GNAI1 refers to the protein produced through recombinant DNA technology, where the GNAI1 gene is cloned and expressed in a suitable host system, such as bacteria or mammalian cells. This allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant GNAI1 is used in various studies to understand its structure, function, and role in cellular signaling pathways.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

GNAI1 Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service