CSF2RA Human, sf9
Colony Stimulating Factor 2 Receptor Alpha Subunit, Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage), Alpha-GM-CSF Receptor, GM-CSF-R-Alpha, CD116 Antigen, GMCSFR-Alpha, GMR-Alpha, CDw116, CSF2RY, CSF2R, Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha, Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain, GM-CSF Receptor Alpha Subunit, AlphaGMR, CSF2RAX, CSF2RAY, CSF2RX, GMCSFR, CD116, SMDP4, GMR.
Greater than 90.0% as determined by SDS-PAGE.
Description
CSF2RA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 310 amino acids (20-320a.a.) and having a molecular mass of 35.9kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). CSF2RA is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The granulocyte-macrophage colony-stimulating factor receptor alpha (CSF2RA), also known as the CD116 antigen, is a protein encoded by the CSF2RA gene in humans. This receptor is crucial for the biological activity of colony-stimulating factor 2 (CSF2), a cytokine that plays a vital role in the production, differentiation, and function of granulocytes and macrophages, which are essential components of the immune system. CSF2RA is a member of the cytokine receptor family and is located on both the X and Y chromosomes in a region known as the pseudoautosomal region (PAR). The gene encoding for CSF2RA exhibits multiple transcript variants, resulting in various isoforms of the protein, some of which are bound to the cell membrane while others are soluble. Dysregulation of CSF2RA has been implicated in various diseases, including surfactant metabolism dysfunction, a condition that affects lung function.
Recombinant CSF2RA protein, expressed in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 310 amino acids, spanning from position 20 to 320 (20-320a.a.), with a molecular weight of 35.9 kDa. On SDS-PAGE, the protein exhibits an apparent molecular weight of approximately 40-57 kDa due to glycosylation. The recombinant CSF2RA protein is engineered with a 9-amino acid Histidine tag (His-tag) at the C-terminus to facilitate purification using proprietary chromatographic techniques.
The CSF2RA protein is supplied as a solution with a concentration of 0.5 mg/ml. The formulation buffer consists of Phosphate Buffered Saline (PBS) at a pH of 7.4 and 10% glycerol.
For short-term storage (up to 2-4 weeks), the CSF2RA protein should be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance long-term stability, adding a carrier protein such as 0.1% HSA (Human Serum Albumin) or BSA (Bovine Serum Albumin) is advisable. Repeated freeze-thaw cycles should be avoided to maintain protein integrity and activity.
The purity of the CSF2RA protein is determined to be greater than 90% by SDS-PAGE analysis.
The biological activity of CSF2RA is evaluated based on its capacity to inhibit the proliferation of TF1 human erythroleukemic cells in a GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) dependent manner. The ED50 value, which represents the effective concentration at which 50% inhibition is observed, is determined to be less than or equal to 10 µg/ml in the presence of 0.5 ng/ml GM-CSF.
Colony Stimulating Factor 2 Receptor Alpha Subunit, Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage), Alpha-GM-CSF Receptor, GM-CSF-R-Alpha, CD116 Antigen, GMCSFR-Alpha, GMR-Alpha, CDw116, CSF2RY, CSF2R, Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha, Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain, GM-CSF Receptor Alpha Subunit, AlphaGMR, CSF2RAX, CSF2RAY, CSF2RX, GMCSFR, CD116, SMDP4, GMR.
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Product Science Overview
The Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha (GM-CSF Receptor Alpha), also known as CSF2RA, is a crucial component in the regulation of white blood cell production, differentiation, and function. This receptor is part of the cytokine receptor family and plays a significant role in the immune system by controlling the production and function of granulocytes and macrophages .
The GM-CSF Receptor Alpha is the alpha subunit of the heterodimeric receptor for colony-stimulating factor 2 (CSF2). It is produced in Sf9 Baculovirus cells and is a single, glycosylated polypeptide chain containing 310 amino acids, with a molecular mass of approximately 35.9 kDa . The receptor is expressed with a 9 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
The gene encoding the GM-CSF Receptor Alpha is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. This gene has multiple transcript variants, some of which are membrane-bound while others are soluble . Diseases associated with CSF2RA include surfactant metabolism dysfunction, pulmonary 4, and CSF2RA-related pulmonary surfactant metabolism dysfunction .
The recombinant form of GM-CSF Receptor Alpha has significant potential in research and therapeutic applications. It is used in studies related to immune response, hematopoiesis, and various diseases associated with the immune system. The availability of this recombinant protein enables researchers to explore its role in different biological processes and develop targeted therapies for related disorders .
The GM-CSF Receptor Alpha protein solution is formulated with phosphate-buffered saline (pH 7.4) and 10% glycerol. For short-term storage, it should be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to freeze the solution at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
