GLUL Antibody
GLNS, EC 6.3.1.2, EC 4.1.1.15, GLUL, Glutamine Synthetase, GS, Glutamate decarboxylase, Glutamate--ammonia ligase, PIG43, PIG59.
Description
Product Specs
Glutamine synthetase (GLUL) is an enzyme responsible for converting glutamate and ammonia into glutamine. Glutamine plays a vital role as an energy source and participates in various cellular processes, including cell proliferation, apoptosis inhibition, and cell signaling. GLUL expression is observed during early fetal development and contributes to maintaining body pH by removing ammonia from circulation. Mutations in the GLUL gene have been linked to congenital glutamine deficiency.
The product appears as a colorless solution that has been sterilized through filtration.
The antibody solution has a concentration of 1mg/ml and is prepared in a buffer containing PBS with a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
For storage durations of up to one month, maintain the product at a temperature of 4°C. For extended storage periods, store at -20°C. It is important to avoid repeated freeze-thaw cycles.
The product remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
The GLUL antibody has been rigorously tested using ELISA and Western blot analysis to ensure its specificity and reactivity. However, as applications may vary, it is recommended to perform reagent titration for each experiment to achieve optimal results.
GLNS, EC 6.3.1.2, EC 4.1.1.15, GLUL, Glutamine Synthetase, GS, Glutamate decarboxylase, Glutamate--ammonia ligase, PIG43, PIG59.
GLUL antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT8D7AT.
Anti-human GLUL mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GLUL amino acids 1-373 purified from E. coli.
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Mouse anti-human antibodies are monoclonal antibodies produced in mice that are specific for human antigens. These antibodies are widely used in research, diagnostics, and therapeutic applications due to their high specificity and affinity for their target antigens .
Production and Applications
The production of mouse anti-human antibodies involves immunizing mice with a specific human antigen, followed by the isolation of B cells that produce antibodies against the antigen. These B cells are then fused with myeloma cells to create hybridomas, which can be cultured to produce large quantities of monoclonal antibodies .
Mouse anti-human antibodies are used in various applications, including:
- Diagnostics: They are used in immunoassays to detect the presence of specific human antigens in clinical samples.
- Research: They are used to study the expression and function of human proteins in various biological processes.
- Therapeutics: Some mouse anti-human antibodies have been developed into therapeutic agents for the treatment of diseases such as cancer and autoimmune disorders .
Human Anti-Mouse Antibody (HAMA) Response
One of the challenges associated with the use of mouse anti-human antibodies in therapeutic applications is the potential for the human immune system to recognize these antibodies as foreign and mount an immune response against them. This response, known as the Human Anti-Mouse Antibody (HAMA) response, can reduce the efficacy of the treatment and cause adverse effects .
To mitigate the HAMA response, various strategies have been developed, including the humanization of mouse antibodies. This involves modifying the antibody’s structure to increase the proportion of human sequences while retaining the specificity and affinity for the target antigen .
