GLRX Mouse
Glutaredoxin-1, Thioltransferase-1, TTase-1, Glrx, Glrx1, Grx, Grx1.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
GLRX Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (1-107 a.a) and having a molecular mass of 14.3kDa. GLRX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Glutaredoxin-1, Thioltransferase-1, TTase-1, Glrx, Glrx1, Grx, Grx1.
MGSSHHHHHH SSGLVPRGSH MGSMAQEFVN CKIQSGKVVV FIKPTCPYCR KTQEILSQLP FKQGLLEFVD ITATNNTSAI QDYLQQLTGA RTVPRVFIGK DCIGGCSDLI SMQQTGELMT RLKQIGALQL
Product Science Overview
Glutaredoxins (GRXs) are small, ubiquitous proteins that play a crucial role in maintaining cellular redox homeostasis. They are part of the thioredoxin superfamily and are involved in various cellular processes, including DNA synthesis, signal transduction, and defense against oxidative stress. This article focuses on the background of mouse recombinant glutaredoxin, highlighting its structure, function, and significance in biological research.
Glutaredoxins are characterized by their conserved active site motif, typically CXXC or CXXS, where “C” stands for cysteine and “X” can be any amino acid. Based on their active sites, GRXs are classified into three subfamilies: CPYC, CGFS, and CC . The CPYC and CGFS subfamilies are present in eukaryotes, while the CC subfamily is exclusive to higher plants .
Recombinant mouse glutaredoxin is commonly expressed in Escherichia coli (E. coli) systems due to the ease of genetic manipulation and high yield of protein production . The recombinant protein is typically purified using affinity chromatography techniques, ensuring high purity and biological activity .
Glutaredoxins are primarily involved in the reduction of disulfide bonds in proteins, utilizing glutathione (GSH) as a cofactor. They play a pivotal role in:
- Redox Homeostasis: Maintaining the balance between oxidation and reduction within the cell.
- DNA Synthesis: Supporting ribonucleotide reductase activity, which is essential for DNA synthesis .
- Signal Transduction: Modulating various signaling pathways by regulating the redox state of target proteins.
- Oxidative Stress Response: Protecting cells from oxidative damage by reducing reactive oxygen species (ROS) and repairing oxidized proteins .
Recombinant mouse glutaredoxin is widely used in research to study its role in cellular processes and its potential therapeutic applications. It serves as a model to understand the function of GRXs in higher organisms and their involvement in diseases such as cancer and neurodegenerative disorders .
