GLRX3 Human
Glutaredoxin-3 Human Recombinant
Cat. No.
BT22024
Source
Escherichia Coli.
Synonyms
Glutaredoxin-3, Thioredoxin-like protein 2, PKC-interacting cousin of thioredoxin,
PKC-theta-interacting protein, PKCq-interacting protein, GLRX3, PICOT, TXNL2, GRX3, GRX4, GLRX4, TXNL3, FLJ11864, bA500G10.4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GLRX3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 a.a.) and having a molecular mass of 39.6kDa. GLRX3 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Glutaredoxin, a member of the thiol-disulfide oxidoreductase family, catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups via a monothiol mechanism. Glutaredoxin-3 (GRX3), a key protein in signal transduction regulation, plays a role in processes like immune cell activation and cardiac hypertrophy development, likely in response to redox signals. It interacts with PRKCQ and may regulate the thioredoxin system's function. GRX3 is primarily found in the heart, spleen, testis, and to a lesser extent in the thymus and peripheral blood leukocytes, with weak expression in the lung, placenta, colon, and small intestine.
Description
Recombinant human GLRX3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 355 amino acids (residues 1-335) with a molecular weight of 39.6 kDa. The protein includes a 20 amino acid His Tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
GLRX3 is supplied in a solution of 20mM Tris-HCl buffer at pH 8.0, with 10% glycerol and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the product is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Glutaredoxin-3, Thioredoxin-like protein 2, PKC-interacting cousin of thioredoxin,
PKC-theta-interacting protein, PKCq-interacting protein, GLRX3, PICOT, TXNL2, GRX3, GRX4, GLRX4, TXNL3, FLJ11864, bA500G10.4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN.
Product Science Overview
Discovery and Structure
Glutaredoxin was first discovered in 1976 by Arne Holmgren. The Glutaredoxin system, which includes Glutaredoxin, glutathione, glutathione reductase, and NADPH, was initially described as an electron donor for Ribonucleotide Reductase . GLRX3 is a small protein containing an active site cysteine pair, which is essential for its redox activity .
Functions and Mechanisms
GLRX3 is involved in various cellular processes, including:
- Redox Regulation: It catalyzes glutathione-dependent redox reactions, such as glutathionylation (conjugation of glutathione to a substrate) and deglutathionylation (removal of glutathione from a substrate) .
- Iron-Sulfur Cluster Formation: GLRX3 plays a role in the maturation of cytosolic iron-sulfur (Fe-S) proteins, which are crucial for various cellular functions .
- Signal Transduction: It is involved in the regulation of signal transduction pathways, particularly during immune cell activation and the development of cardiac hypertrophy .
Recombinant Human Glutaredoxin-3
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